Transcriptome analysis in Parhyale hawaiensis reveal sex-specific responses to AgNP and AgCl exposure

Autor(es)

Artal, Mariana Coletty ^{[1, 2]} ; Pereira, Karina Danielle ^{[3, 4]} ; Luchessi, Augusto Ducati ^{[3, 4]} ; Okura, Vagner Katsumi ^[5] ; Henry, Theodore Burdick ^{[6, 7]} ; Marques-Souza, Henrique ^[8] ; Umbuzeiro, Gisela de Aragao ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Sch Technol, BR-13484332 Limeira, SP - Brazil

^[2] Univ Sao Paulo, Sch Pharmaceut Sci, BR-05508000 Sao Paulo - Brazil

^[3] Sao Paulo State Univ, Inst Biosci, BR-13506900 Rio Claro, SP - Brazil

^[4] Univ Estadual Campinas, Sch Appl Sci, Lab Biotechnol, BR-13484350 Limeira, SP - Brazil

^[5] Univ Estadual Campinas, Life Sci Core Facil LaCTAD, BR-13083886 Campinas, SP - Brazil

^[6] Univ Tennessee, Ctr Environm Biotechnol, 676 Dabney Hall, 1416 Circle Dr, Knoxville, TN 37996 - USA

^[7] Heriot Watt Univ, Sch Energy Geosci Infrastruct & Soc, Inst Life & Earth Sci, Edinburgh EH14 4AS, Midlothian - Scotland

^[8] Univ Estadual Campinas, Dept Biochem & Tissue Biol, BR-13083970 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Environmental Pollution; v. 260, MAY 2020.
• Citações Web of Science: 1

Resumo

Analysis of the transcriptome of organisms exposed to toxicants offers new insights for ecotoxicology, but further research is needed to enhance interpretation of results and effectively incorporate them into useful environmental risk assessments. Factors that must be clarified to improve use of transcriptomics include assessment of the effect of organism sex within the context of toxicant exposure. Amphipods are well recognized as model organisms for toxicity evaluation because of their sensitivity and amenability to laboratory conditions. To investigate whether response to metals in crustaceans differs according to sex we analyzed the amphipod Parhyale hawaiensis after exposure to AgCl and Ag nanoparticles (AgNP) via contaminated food. Gene specific analysis and whole genome transcriptional profile of male and female organisms were performed by both RT-qPCR and RNA-seq. We observed that expression of transcripts of genes glutathione transferase (GST) did not differ among AgCl and AgNP treatments. Significant differences between males and females were observed after exposure to AgCl and AgNP. Males presented twice the number of differentially expressed genes in comparison to females, and more differentially expressed were observed after exposure to AgNP than AgCl treatments in both sexes. The genes that had the greatest change in expression relative to control were those genes related to peptidase and catalytic activity and chitin and carbohydrate metabolic processes. Our study is the first to demonstrate sex specific differences in the transcriptomes of amphipods upon exposure to toxicants and emphasizes the importance of considering gender in ecotoxicology. (C) 2020 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 14/08829-7 - Desenvolvimento de biomarcadores de ecotoxicidade no anfípoda marinho Parhyale hawaiensis e avaliação da toxicidade de nanomateriais
• Beneficiário: Mariana Coletty Artal
• Modalidade de apoio: Bolsas no Brasil - Doutorado