Phenotypic traits of Burkholderia spp. associated with ecological adaptation and plant-host interaction

Autor(es)

Romero-Gutierrez, Karent J. ^[1] ; Dourado, Manuella N. ^[1] ; Garrido, Leandro M. ^[1] ; Olchanheski, Luiz Ricardo ^[1] ; Mano, Emy T. ^[1] ; Dini-Andreote, Francisco ^{[2, 3]} ; Valvano, Miguel A. ^[4] ; Araujo, Welington L. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, Av Prof Lineu Presses, 1374 Biomed II, Cidade Univ, Sao Paulo 05508900, SP - Brazil

^[2] Penn State Univ, Dept Plant Sci, University Pk, PA 16802 - USA

^[3] Penn State Univ, Huck Inst Life Sci, University Pk, PA 16802 - USA

^[4] Queens Univ Belfast, Wellcome Wolfson Inst Expt Med, Belfast BT9 7BL, Antrim - North Ireland

• Tipo de documento: Artigo Científico
• Fonte: MICROBIOLOGICAL RESEARCH; v. 236, JUN 2020.
• Citações Web of Science: 0

Resumo

Burkholderia species have different lifestyles establishing mutualist or pathogenic associations with plants and animals. Changes in the ecological behavior of these bacteria may depend on genetic variations in response to niche adaptation. Here, we studied 15 Burkholderia strains isolated from different environments with respect to genetic and phenotypic traits. By Multilocus Sequence Analysis (MLSA) these isolates fell into 6 distinct groups. MLSA clusters did not correlate with strain antibiotic sensitivity, but with the bacterial ability to produce antimicrobial compounds and control orchid necrosis. Further, the B. seminalis strain TC3.4.2R3, a mutualistic bacterium, was inoculated into orchid plants and the interaction with the host was evaluated by analyzing the plant response and the bacterial oxidative stress response in planta. TC3.4.2R3 responded to plant colonization by increasing its own growth rate and by differential gene regulation upon oxidative stress caused by the plant, while reducing the plant's membrane lipid peroxidation. The bacterial responses to oxidative stress were recapitulated by bacterial exposure to the herbicide paraquat. We suggest that the ability of Burkholderia species to successfully establish in the rhizosphere correlates with genetic variation, whereas traits associated with antibiotic resistance are more likely to be categorized as strain specific. (AU)

• Processo FAPESP: 17/12510-4 - Micro-organismos endofíticos e a planta hospedeira: genes e metabólitos envolvidos na interação
• Beneficiário: Welington Luiz de Araújo
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/11271-0 - Prospecção, clonagem e expressão de genes de Burkholderia sp. com potencial para o controle de fitopatógenos
• Beneficiário: Emy Tiyo Mano
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 12/24217-6 - Micro-organismos endofíticos: identificação de genes envolvidos em interações microbianas
• Beneficiário: Welington Luiz de Araújo
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 18/22127-6 - Burkholderia spp. e o microbioma associado: os mecanismos de interação na rizosfera e nos pulmões são similares?
• Beneficiário: Welington Luiz de Araújo
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 15/11563-1 - Mecanismos moleculares envolvidos na interação entre micro-organismos endofíticos e a planta hospedeira
• Beneficiário: Welington Luiz de Araújo
• Modalidade de apoio: Auxílio à Pesquisa - Regular