Genome constellations of rotavirus a isolated from avian species in Brazil, 2008-2015

Autor(es)

Beserra, Laila A. R. ^[1] ; Barbosa, Carla M. ^[2] ; Berg, Mikael ^[3] ; Brandao, Paulo E. ^[1] ; Soares, Rodrigo M. ^[1] ; Gregori, Fabio ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Vet Med, Dept Prevent Vet Med & Anim Hlth, Av Prof Dr Orlando Marques de Paiva 87, BR-05508270 Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Inst Biomed Sci, Av Prof Dr Prof Lineu Prestes 1374, BR-05508900 Sao Paulo, SP - Brazil

^[3] Swedish Univ Agr Sci SLU BVF, Dept Biomed Sci & Vet Publ Hlth, Box 7028, S-75007 Uppsala - Sweden

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Journal of Microbiology; v. 51, n. 3 MAY 2020.
• Citações Web of Science: 0

Resumo

Rotaviruses are members of the family Reoviridae and are a common cause of acute diarrhea in many mammalian and avian species. They are non-enveloped icosahedral particles and their genome comprises 11 segments of double-stranded RNA, which encodes six structural proteins (VP1-4, VP6-7) and six nonstructural proteins (NSP1-6). Genotypes are defined based upon the diversity found in these genes and viral characterization plays a central role on epidemiological studies and prevention. Here we investigate the distribution of Brazilian RVAs genotypes in 8 chicken samples collected between 2008 and 2015 from different regions by RT-PCR, partial (Sanger) nucleotide sequencing and phylogenetic analysis from all rotavirus genes. Although the identified genotypes were typical from avian host species, when analyzed together, they form novel genetic constellations: G19-P{[}31]-I11-R6-C6-M7-A16-N6-T8-E10-H8 and G19-P{[}31]-I4-R4-C4-M4-A16-N4-T4-E4-H4. This study highlights that avian rotaviruses are widespread among commercial farms in Brazil, and the co-circulation of at least two different genomic constellations indicates that may present a way bigger genetic variability, that can be increased by the possible transmission events from other birds, lack of specific preventive measures, as well as the different viral evolution mechanisms. (AU)

• Processo FAPESP: 14/13531-7 - Análise multigênica de rotavírus do grupo a em aves de criações comerciais brasileiras
• Beneficiário: Fábio Gregori
• Modalidade de apoio: Auxílio à Pesquisa - Regular