Recombinant expression, characterization and phylogenetic studies of novels cystatins-like proteins of sweet orange (Citrus sinensis) and clementine (Citrus clementina)

Autor(es):Mostrar menos -

Schneider, Vanessa Karine ^[1] ; da Silva Ferrara, Taise Fernanda ^[2] ; Rocha, Samara Vieira ^[1] ; Santos-Junior, Celio Dias ^[1] ; Neo-Justino, Daniela Morilha ^[1] ; da Cunha, Anderson Ferreira ^[1] ; Maia de Oliveira Silva, Joao Pedro ^[1] ; dos Santos Tersariol, Ivarne Luis ^[3] ; Carmona, Adriana Karaoglanovic ^[2] ; Henrique-Silva, Flavio ^[1] ; Soares-Costa, Andrea ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos, Dept Genet & Evolucao, Sao Paulo - Brazil

^[2] Univ Fed Sao Paulo, Dept Biofis, Sao Paulo - Brazil

^[3] Univ Fed Sao Paulo, Dept Bioquim, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: International Journal of Biological Macromolecules; v. 152, p. 546-553, JUN 1 2020.
• Citações Web of Science: 0

Resumo

Phytocystatins are plant cystatins that are related to several physiological processes regulating endogenous cysteine proteases involved in seed development and germination, programmed cell death and response to stress conditions. In addition, phytocystatins can act in plant defense against exogenous peptidases from herbivorous insects, pathogens and nematodes. Considering that Citrus fruits are important to human nutrition and represent a high value crop in worldwide agriculture, in the present work, we performed the identification of putative cystatins from Citrus sinensis and from Citrus clementine and submitted them to phylogenetic analysis. Six cystatins from each species were identified as orthologous and classified into three well supported phylogenetic groups. Five cystatins representative of the phylogenetic groups were recombinantly expressed and the in vitro studies revealed them to be potent inhibitors against the cysteine peptidases papain, legumain, human cathepsins (B, L S. K) and a cathepsin B-like from Diaphorina citri (the Asian Citrus psyllid). Our findings provide the C. clementina and C. sinensis cystatins classification and an enzyme-inhibitor interactions profile, which may reflect an evolutionary process of Grills cystatins related to gene functions as initial germination rates and seedlings development as well associated to plant defense against pathogens, as insects and nematodes. (C) 2020 Elsevier B.V. All lights reserved. (AU)

• Processo FAPESP: 16/02412-2 - Identificação e caracterização de alvos para controle do inseto Diaphorina citri, vetor da doença do citros Huanglongbing
• Beneficiário: Taíse Fernanda da Silva Ferrara
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 13/05370-0 - Desenvolvimento de linhagens de cana-de-açúcar transgênica superexpressando inibidores de peptidases visando resistência ao inseto Migdolus fryanus
• Beneficiário: Vanessa Karine Schneider
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto