Recombinant expression, characterization and phylogenetic studies of novels cystatins-like proteins of sweet orange (Citrus sinensis) and clementine (Citrus clementina)

Author(s):Show less -

Schneider, Vanessa Karine ^[1] ; da Silva Ferrara, Taise Fernanda ^[2] ; Rocha, Samara Vieira ^[1] ; Santos-Junior, Celio Dias ^[1] ; Neo-Justino, Daniela Morilha ^[1] ; da Cunha, Anderson Ferreira ^[1] ; Maia de Oliveira Silva, Joao Pedro ^[1] ; dos Santos Tersariol, Ivarne Luis ^[3] ; Carmona, Adriana Karaoglanovic ^[2] ; Henrique-Silva, Flavio ^[1] ; Soares-Costa, Andrea ^[1]

Affiliation

^[1] Univ Fed Sao Carlos, Dept Genet & Evolucao, Sao Paulo - Brazil

^[2] Univ Fed Sao Paulo, Dept Biofis, Sao Paulo - Brazil

^[3] Univ Fed Sao Paulo, Dept Bioquim, Sao Paulo - Brazil

• Document type: Journal article
• Source: International Journal of Biological Macromolecules; v. 152, p. 546-553, JUN 1 2020.
• Web of Science Citations: 0

Abstract

Phytocystatins are plant cystatins that are related to several physiological processes regulating endogenous cysteine proteases involved in seed development and germination, programmed cell death and response to stress conditions. In addition, phytocystatins can act in plant defense against exogenous peptidases from herbivorous insects, pathogens and nematodes. Considering that Citrus fruits are important to human nutrition and represent a high value crop in worldwide agriculture, in the present work, we performed the identification of putative cystatins from Citrus sinensis and from Citrus clementine and submitted them to phylogenetic analysis. Six cystatins from each species were identified as orthologous and classified into three well supported phylogenetic groups. Five cystatins representative of the phylogenetic groups were recombinantly expressed and the in vitro studies revealed them to be potent inhibitors against the cysteine peptidases papain, legumain, human cathepsins (B, L S. K) and a cathepsin B-like from Diaphorina citri (the Asian Citrus psyllid). Our findings provide the C. clementina and C. sinensis cystatins classification and an enzyme-inhibitor interactions profile, which may reflect an evolutionary process of Grills cystatins related to gene functions as initial germination rates and seedlings development as well associated to plant defense against pathogens, as insects and nematodes. (C) 2020 Elsevier B.V. All lights reserved. (AU)

• FAPESP's process: 16/02412-2 - Identification and characterization of targets for insect control Diaphorina citri, vector citrus disease Huanglongbing
• Grantee: Taíse Fernanda da Silva Ferrara
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 13/05370-0 - Development of transgenic sugarcane strains overexpressing peptidase inhibitors for resistance to insect Migdolus fryanus
• Grantee: Vanessa Karine Schneider
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)