DNA methylation pattern changes following a short-term hypocaloric diet in women with obesity

Autor(es)

Nicoletti, C. F. ^{[1, 2]} ; Cortes-Oliveira, C. ^[1] ; Noronha, N. Y. ^[1] ; Pinhel, M. A. S. ^{[1, 3]} ; Dantas, W. S. ^[2] ; Jacome, A. ^[4] ; Marchini, J. S. ^[5] ; Gualano, B. ^[2] ; Crujeiras, A. B. ^{[6, 7]} ; Nonino, C. B. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Hlth Sci, Lab Nutrigen Studies, Ribeirao Preto - Brazil

^[2] Univ Sao Paulo, Fac Med FMUSP, Sch Phys Educaton & Sport, Rheumatol Div, Appl Physiol & Nutr Res Grp, Sao Paulo, SP - Brazil

^[3] Sao Jose do Rio Preto Med Sch, Dept Mol Biol, Lab Studies Biochem & Mol Biol, Sao Paulo - Brazil

^[4] Univ A Coruna, Fac Sci, Dept Math, MODES Grp, CITIC, La Coruna - Spain

^[5] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Internal Med, Ribeirao Preto - Brazil

^[6] CIBER Fisiopatol Obesidad & Nutr CIBERobn, Madrid - Spain

^[7] Santiago de Compostela Univ USC, Complejo Hosp Univ Santiago CHUS, Inst Invest Sanitaria IDIS, Epigen Endocrinol & Nutr, Santiago De Compostela - Spain

• Tipo de documento: Artigo Científico
• Fonte: European Journal of Clinical Nutrition; v. 74, n. 9 MAY 2020.
• Citações Web of Science: 3

Resumo

Background/Objectives We aimed to investigate the effects of short-term hypocaloric diet-induced weight loss on DNA methylation profile in leukocytes from women with severe obesity. Methods Eleven women with morbid obesity (age: 36.9 +/- 10.3 years; BMI: 58.5 +/- 10.5 kg/m(2)) were assessed before and after 6 weeks of a hypocaloric dietary intervention. The participants were compared with women of average weight and the same age (age: 36.9 +/- 11.8 years; BMI: 22.5 +/- 1.6 kg/m(2)). Genome-wide DNA methylation analysis was performed in DNA extracted from peripheral blood leukocytes using the Infinium Human Methylation 450 BeadChip assay. Changes (Delta beta) in the methylation level of each CpGs were calculated. A threshold with a minimum value of 10%, p < 0.001, for the significant CpG sites based on Delta beta and a false discovery rate of Results Dietary intervention changed the methylation levels at 16,064 CpG sites. These CpGs sites were related to cancer, cell cycle-related, MAPK, Rap1, and Ras signaling pathways. However, regardless of hypocaloric intervention, a group of 878 CpGs (related to 649 genes) remained significantly altered in obese women when compared with normal-weight women. Pathway enrichment analysis identified genes related to the cadherin and Wnt pathway, angiogenesis signaling, and p53 pathways by glucose deprivation. Conclusion A short-term hypocaloric intervention in patients with severe obesity partially restored the obesity-related DNA methylation pattern. Thus, the full change of obesity-related DNA methylation patterns could be proportional to the weight-loss rate in these patients after dietary interventions. (AU)

• Processo FAPESP: 16/05638-1 - Padrão de metilação global do DNA antes e após tratamentos para perda de peso
• Beneficiário: Carla Barbosa Nonino
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/06470-7 - Identificação de marcadores genéticos associados com as respostas metabólicas após intervenção dietética hipocalórica em mulheres com obesidade grau III
• Beneficiário: Cristiana Cortes de Oliveira
• Modalidade de apoio: Bolsas no Brasil - Doutorado