A MODIFIED MOUSE-TAIL LYMPHEDEMA MODEL

Autor(es)

Arruda, G. ^[1] ; Ariga, S. ^[1] ; de Lima, T. M. ^[1] ; Souza, H. P. ^[1] ; Andrade, M. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Emergency Dept LIM 51, Fac Med, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Dept Surg LIM 02, Fac Med, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: LYMPHOLOGY; v. 53, n. 1, p. 29-37, MAR 2020.
• Citações Web of Science: 0

Resumo

One of the main obstacles to studying the pathophysiology of lymphedema development is the lack of appropriate experimental models. Following up on a mouse-tail method that has been described, we performed changes to the method which made it easier to perform in our hands and demonstrated similar results. Twenty C57Black mice were operated on using the previous technique and euthanized after 3 or 6 weeks. Another twenty mice were submitted to the new technique developed in our laboratory and euthanized at the same time points. Tissue samples were collected from the proximal part of the tail (control) and from the distal part (lymphedema) for both models. Animals in both operative groups developed marked edema in the distal part of the tail. This was characterized by lymph vessels dilation, edema, inflammatory cell infiltration, and adipose tissue deposition. Lymphedema was detected after 3 weeks in both models, reaching its maximum after 6 weeks. Adipocytes detected by histology (Oil red O staining) and molecular markers for adipogenesis, lymphangiogenesis and inflammation (lipin 1 and 2, SLP76, and F4-80) were demonstrated to be increased equally in both models. In conclusion, both models provide a reliable method to study lymphedema pathophysiology. However; our modified technique is easier and faster to perform while still providing reliable and consistent results. (AU)

• Processo FAPESP: 17/05232-8 - Interações entre inflamação e obesidade: mecanismos pelos quais o processo inflamatório influencia a adipogênese e as complicações da obesidade
• Beneficiário: Heraldo Possolo de Souza
• Modalidade de apoio: Auxílio à Pesquisa - Regular