Environmental safety and mode of action of a novel curcumin-based photolarvicide

Autor(es)

Venturini, Francine Perri ^{[1, 2]} ; de Souza, Larissa Marila ^{[3, 2]} ; Garbuio, Matheus ^{[3, 2]} ; Inada, Natalia Mayumi ^[2] ; de Souza, Jaqueline Perola ^[1] ; Kurachi, Cristina ^[2] ; de Oliveira, Kleber Thiago ^[4] ; Bagnato, Vanderlei Salvador ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Nanomed & Nanotoxicol Grp, Sao Carlos Inst Phys, BR-13560970 Sao Carlos, SP - Brazil

^[2] Univ Sao Paulo, Sao Carlos Inst Phys, Opt Grp, BR-13560970 Sao Carlos, SP - Brazil

^[3] Univ Fed Sao Carlos, PPG Biotec, BR-13565905 Sao Carlos, SP - Brazil

^[4] Univ Fed Sao Carlos, Dept Chem, BR-13565905 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Environmental Science and Pollution Research; v. 27, n. 23 MAY 2020.
• Citações Web of Science: 0

Resumo

Aedes aegypti is the vector of important diseases like dengue, zika, chikungunya, and yellow fever. Vector control is pivotal in combating the spread of these mosquito-borne illnesses. Photoactivable larvicide curcumin obtained from Curcuma longa Linnaeus has shown high potential for Ae. aegypti larvae control. However, the toxicity of this photosensitizer (PS) might jeopardize non-target aquatic organisms. The aim of this study was to evaluate the toxicity of this PS to Daphnia magna and Danio rerio, besides assessing its mode of action through larvae biochemical and histological studies. Three PS formulations were tested: PS in ethanol+DMSO, PS in sucrose, and PS in D-mannitol. The LC50 of PS in ethanol+DMSO to D. rerio was 5.9 mg L-1, while in D. magna the solvents were extremely toxic, and LC50 was not estimated. The PS formulations in sugars were not toxic to neither of the organisms. Reactive oxygen species (ROS) were generated in D. magna exposed to 50 mg L-1 of PS in D-mannitol, and D. rerio did not elicit this kind of response. D. magna feeding rates were not affected by the PS in D-mannitol. Concerning Ae. aegypti larvae, there were changes in reduced glutathione and protein levels, while catalase activity remained stable after exposure to PS in D-mannitol and sunlight. Histological changes were observed in larvae exposed to PS in sucrose and D-mannitol, most of them irreversible and deleterious. Our results show the feasibility of this photolarvicide use in Ae. aegypti larvae control and its safety to non-target organisms. These data are crucial to this original vector control approach implementation in public health policies. (AU)

• Processo FAPESP: 18/00106-7 - Reações Fotocatalisadas por Porfirinas em Condições de Fluxo Contínuo: Arilação de Sistemas Heterocíclicos e de Olefinas Ativadas
• Beneficiário: Kleber Thiago de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/07276-1 - CEPOF - Centro de Pesquisa em Óptica e Fotônica
• Beneficiário: Vanderlei Salvador Bagnato
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs