| Texto completo | |
| Autor(es): Mostrar menos - |
Griffiths, Victoria A.
[1]
;
Valera, Antoine M.
[1]
;
Lau, Joanna Y. N.
[1]
;
Ros, Hana
[1]
;
Younts, Thomas J.
[1]
;
Marin, Boris
[1, 2]
;
Baragli, Chiara
[1]
;
Coyle, Diccon
[1]
;
Evans, Geoffrey J.
[1, 3]
;
Konstantinou, George
[4, 1]
;
Koimtzis, Theo
[1, 5]
;
Nadella, K. M. Naga Srinivas
[1]
;
Punde, Sameer A.
[1]
;
Kirkby, Paul A.
[1]
;
Bianco, Isaac H.
[1]
;
Silver, R. Angus
[1]
Número total de Autores: 16
|
| Afiliação do(s) autor(es): | [1] UCL, Dept Neurosci Physiol & Pharmacol, London - England
[2] Univ Fed ABC, Ctr Matemat Comp & Cognicao, Sao Bernardo Do Campo - Brazil
[3] Sencon UK Ltd, Dept Engn, Droitwich - England
[4] Francis Crick Inst, London - England
[5] Opt Metrol Serv, Stansted - England
Número total de Afiliações: 5
|
| Tipo de documento: | Artigo Científico |
| Fonte: | NATURE METHODS; v. 17, n. 7 JUN 2020. |
| Citações Web of Science: | 0 |
| Resumo | |
Two-photon microscopy is widely used to investigate brain function across multiple spatial scales. However, measurements of neural activity are compromised by brain movement in behaving animals. Brain motion-induced artifacts are typically corrected using post hoc processing of two-dimensional images, but this approach is slow and does not correct for axial movements. Moreover, the deleterious effects of brain movement on high-speed imaging of small regions of interest and photostimulation cannot be corrected post hoc. To address this problem, we combined random-access three-dimensional (3D) laser scanning using an acousto-optic lens and rapid closed-loop field programmable gate array processing to track 3D brain movement and correct motion artifacts in real time at up to 1 kHz. Our recordings from synapses, dendrites and large neuronal populations in behaving mice and zebrafish demonstrate real-time movement-corrected 3D two-photon imaging with submicrometer precision. Real-time 3D movement correction by tracking a fluorescent bead in the field of view enables functional imaging with 3D two-photon random-access microscopy in behaving mice and zebrafish. (AU) | |
| Processo FAPESP: | 18/20277-0 - Neurociência computacional e de sistemas |
| Beneficiário: | Antonio Carlos Roque da Silva Filho |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |