Mechanical-chemical coupling in Temporomandibular Joint disc

Autor(es)

do Nascimento, Rodney Marcelo ^{[1, 2, 3]} ; Baldit, Adrien ^[3] ; Kokanyan, Ninel ^{[4, 5]} ; Tappert, Lara Kristin ^[3] ; Lipinski, Paul ^[3] ; Hernandes, Antonio Carlos ^[2] ; Rahouadj, Rachid ^[3]

Afiliação do(s) autor(es)

^[1] Univ Fed Santa Catarina, Dept Fis, BR-88040900 Florianopolis, SC - Brazil

^[2] Univ Sao Paulo, Sao Carlos Inst Phys, BR-13566590 Sao Paulo - Brazil

^[3] Univ Lorraine Nancy Metz, Lab Etud Microstruct Mecan Mat & Vivant, LEM3 UMR CNRS 7239, F-57070 Nancy - France

^[4] Univ Paris Saclay, Lab Mat Opt Photon & Syst LMOPS, Cent Supelec, 2 Rue E, F-57070 Metz - France

^[5] Univ Lorraine, Lab Mat Opt Photon & Syst LMOPS, EA 4423, 2 Rue E Belin, F-57070 Metz - France

• Tipo de documento: Artigo Científico
• Fonte: MATERIALIA; v. 9, MAR 2020.
• Citações Web of Science: 0

Resumo

This paper reports an experimental investigation based on vibrational spectroscopy of biological materials by a new custom-made device allowing simultaneously applying tensile load with changes within a controlled chemical environment. The response of the molecular structures of Temporomandibular Joint (TMJ) discs under simulated daily mechanical stimulations was characterized by stress-strain analysis and Raman spectra. The results show that changes in the biochemical environment around tissue (associated to common disorders) led to significant modifications to its mechanical properties. The molecular response to stress was then fully characterized by a combination of molecular mapping followed by statistical analysis via Principal Component Analysis resulting in the identification of the phenylalanine-aromatic amino acids as a significant mechanical-chemical effector of TMJ discs. The coupled tensile machine and vibrational spectroscopy approach enabling in-situ molecular studies proves to be a powerful technique for biological material characterization and tissue engineering. (AU)

• Processo FAPESP: 17/03842-3 - Estudo da Regeneração Tecidual Guiada (GTR) através de análise experimental em disco articular temporo-mandibular e membranas oclusivas NR-CaP
• Beneficiário: Rodney Marcelo do Nascimento
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado
• Processo FAPESP: 13/21970-8 - Desenvolvimento de biomaterial a partir da incorporação de fosfatos de cálcio em estruturas de látex visando aplicação como dispositivo de liberação controlada
• Beneficiário: Rodney Marcelo do Nascimento
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado