The Ex Vivo Skin Model as an Alternative Tool for the Efficacy and Safety Evaluation of Topical Products

Autor(es)

Eberlin, Samara ^[1] ; da Silva, Michelle Sabrina ^{[1, 2]} ; Facchini, Gustavo ^{[1, 2]} ; da Silva, Gustavo Henrique ^{[1, 2]} ; Tabarini Alves Pinheiro, Ana Lucia ^{[1, 2]} ; Eberlin, Samir ^[3] ; Pinheiro, Adriano da Silva ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Kosmosci Grp, Rua Sandoval Meirelles 72, BR-13040315 Campinas, SP - Brazil

^[2] Eberlin, Samir, Sante DOr Inst, Sumare, SP, Brazil.Eberlin, Samara, Kosmosci Grp, Rua Sandoval Meirelles 72, BR-13040315 Campinas, SP - Brazil

^[3] Sante DOr Inst, Sumare, SP - Brazil

• Tipo de documento: Artigo de Revisão
• Fonte: ATLA-ALTERNATIVES TO LABORATORY ANIMALS; v. 48, n. 1, p. 10-22, JAN 2020.
• Citações Web of Science: 4

Resumo

The development of alternative approaches for safety and efficacy testing that avoid the use of animals is a worldwide trend, which relies on the improvement of current models and tools so that they better reproduce human biology. Human skin from elective plastic surgery is a promising experimental model to test the effects of topically applied products. As the structure of native skin is maintained, including cell population (keratinocytes, melanocytes, Langerhans cells and fibroblasts) and dermal matrix (containing collagen, elastin, glycosaminoglycans, etc.), it most closely matches the effects of substances on in vivo human skin. In this review, we present a collection of results that our group has generated over the last years, involving the use of human skin and scalp explants, demonstrating the feasibility of this model. The development of a test system with ex vivo skin explants, of standard size and thickness, and cultured at the air-liquid interface, can provide an important tool for understanding the mechanisms involved in several cutaneous disorders. (AU)

• Processo FAPESP: 17/15935-6 - Padronização de modelo de pele humana ex vivo para ensaios alternativos na avaliação do potencial de irritação e corrosão da pele
• Beneficiário: Samara Eberlin
• Modalidade de apoio: Auxílio à Pesquisa - Pesquisa Inovativa em Pequenas Empresas - PIPE
• Processo FAPESP: 15/08598-8 - Determinação dos efeitos da radiação infravermelha-A sobre a modulação gênica em cultura de pele humana para desenvolvimento de uma metodologia alternativa de avaliação de eficácia pré-clínica
• Beneficiário: Samara Eberlin
• Modalidade de apoio: Auxílio à Pesquisa - Pesquisa Inovativa em Pequenas Empresas - PIPE