Phylogeny of Anopheles (Kerteszia) (Diptera: Culicidae) Using Mitochondrial Genes

Autor(es)

Kirchgatter, Karin ^[1] ; de Oliveira Guimaraes, Lilian ^{[1, 2]} ; Yanez Trujillano, Henrry Hugo ^[3] ; Rafael Arias, Fernando ^[4] ; German Caceres, Abraham ^{[5, 6, 7]} ; Ribeiro de Castro Duarte, Ana Maria ^{[1, 2]} ; Malafronte, Rosely dos Santos ^[8] ; Tubaki, Rosa Maria ^[9] ; Mureb Sallum, Maria Anice ^[10]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Superintendencia Controle Endemias, Lab Bioquim & Biol Mol, Inst Med Trop Sao Paulo, BR-01027000 Sao Paulo, SP - Brazil

^[2] Mureb Sallum, Maria Anice, Univ Sao Paulo, Fac Saude Publ, Dept Epidemiol, BR-01246904 Sao Paulo, SP, Brazil.Kirchgatter, Karin, Univ Sao Paulo, Superintendencia Controle Endemias, Lab Bioquim & Biol Mol, Inst Med Trop Sao Paulo, BR-01027000 Sao Paulo, SP - Brazil

^[3] Direcc Reg Salud Cusco, Direcc Ejecut Salud Ambiental, Direcc Salud Ambiental, Area Vigilancia & Control Vectorial, Cuzco 08003 - Peru

^[4] Direcc Reg Salud Puno, Lab Referencia Reg Salud Publ, Puno 21002 - Peru

^[5] Univ Nacl Mayor San Marcos, Fac Med, Inst Med Trop Daniel A Carrion, Secc Entomol, Lima 15081 - Peru

^[6] Univ Nacl Mayor San Marcos, Fac Med, Dept Acad Microbiol Med, Lima 15081 - Peru

^[7] Inst Nacl Salud, Lab Entomol, Lima 15064 - Peru

^[8] Univ Sao Paulo, Inst Med Trop Sao Paulo, Lab Protozool, BR-05403000 Sao Paulo, SP - Brazil

^[9] Superintendencia Controle Endemias Estado Sao Paul, Lab Entomol Med, BR-01027000 Sao Paulo, SP - Brazil

^[10] Univ Sao Paulo, Fac Saude Publ, Dept Epidemiol, BR-01246904 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: INSECTS; v. 11, n. 5 MAY 2020.
• Citações Web of Science: 0

Resumo

Identification of mosquito species is necessary for determining the entomological components of malaria transmission, but it can be difficult in morphologically similar species. DNA sequences are largely used as an additional tool for species recognition, including those that belong to species complexes. Kerteszia mosquitoes are vectors of human and simian malaria in the Neotropical Region, but there are few DNA sequences of Kerteszia species in public databases. In order to provide relevant information about diversity and improve knowledge in taxonomy of Kerteszia species in Peru, we sequenced part of the mitochondrial genome, including the cytochrome c oxidase I (COI) barcode region. Phylogenetic analyses structured all species of mosquitoes collected in Peru into a single clade, separate from the Brazilian species. The Peruvian clade was composed of two lineages, encompassing sequences from Anopheles (Kerteszia) boliviensis and Anopheles (Kerteszia) pholidotus. An. pholidotus sequences were recorded for the first time in Peru, whereas An. boliviensis sequences were for the first time published in the GenBank database. Sequences generated from specimens morphologically identified as Anopheles (Kerteszia) cruzii clustered into three separate clades according to the collection localities of Serra do Mar, Serra da Mantiqueira, and Serra da Cantareira, confirming An. cruzii as a species complex, composed of at least three putative species. (AU)

• Processo FAPESP: 14/26229-7 - Genômica de paisagens em gradientes latitudinais e ecologia de Anopheles darlingi
• Beneficiário: Maria Anice Mureb Sallum
• Linha de fomento: Auxílio à Pesquisa - Temático
• Processo FAPESP: 08/52016-0 - Estudo da fauna de anofelinos e de sua infecção natural por Plasmodium sp. em área de foco de malária autóctone em Parelheiros, Grande São Paulo
• Beneficiário: Ana Maria Ribeiro de Castro Duarte
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 18/16232-1 - Hemosporídeos e arbovírus emergentes em culicídeos e culicoides do Parque Zoológico de São Paulo
• Beneficiário: Lilian de Oliveira Guimarães
• Linha de fomento: Bolsas no Brasil - Pós-Doutorado