Plasma Electrolytic Oxidation as a Feasible Surface Treatment for Biomedical Applications: an in vivo study

Autor(es):Mostrar menos -

Braga Polo, Tarik Okon ^[1] ; da Silva, William Phillip ^[1] ; Correa Momesso, Gustavo Antonio ^[1] ; Lima-Neto, Tiburtino Jose ^[1] ; Barbosa, Stefany ^[2] ; Cordeiro, Jairo Matozinho ^{[3, 4]} ; Hassumi, Jaqueline Suemi ^[1] ; da Cruz, Nilson Cristino ^[5] ; Okamoto, Roberta ^[6] ; Barao, Valentim A. R. ^{[3, 4]} ; Faverani, Leonardo P. ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ Unesp, Sch Dent, Dept Diag & Surg, Rua Jose Bonifacio 1193, BR-16015050 Aracatuba, SP - Brazil

^[2] Sao Paulo State Univ Unesp, Sch Dent, Rua Jose Bonifacio 1193, BR-16015050 Aracatuba, SP - Brazil

^[3] Univ Campinas UNICAMP, Piracicaba Dent Sch, Dept Prosthodont & Periodontol, Av Limeira 901, BR-13414903 Piracicaba, SP - Brazil

^[4] Inst Biomat Tribocorros & Nanomed IBTN, Sao Paulo - Brazil

^[5] Sao Paulo State Univ UNESP, Technol Plasma Lab LaPTec, Expt Campus Sorocaba, Sorocaba - Brazil

^[6] Sao Paulo State Univ UNESP, Dept Basic Sci, Sch Dent, Rua Jose Bonifacio 1193, BR-16015050 Aracatuba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 10, n. 1 JUN 19 2020.
• Citações Web of Science: 0

Resumo

Objectives: In this in vivo animal study, we evaluated the effect of plasma electrolytic oxidation (PEO) coating on the topographic and biological parameters of implants installed in rats with induced osteoporosis and low-quality bones. Materials and methods: In total 44 Wistar rats (Rattus novergicus), 6 months old, were submitted to ovariectomy (OXV group) and dummy surgery (SHAM group). After 90 days, the ELISA test was performed and the ovariectomy effectiveness was confirmed. In each tibial metaphysis, an implant with PEO coating containing Ca2+ and P5+ molecules were installed, and the other tibia received an implant with SLA acid etching and blasting (AC) (control surface). After 42 days, 16 rats from each group were euthanized, their tibias were removed for histological and immunohistochemical analysis (OPG, RANKL, OC and TRAP), as well as reverse torque biomechanics. Data were submitted to One-way ANOVA or Kruskal-Wallis tests, followed by a Tukey post-test; P<0.05. Histological analyses showed higher bone neoformation values among the members of the PEO group, SHAM and OVX groups. Immunohistochemical analysis demonstrated equilibrium in all groups when comparing surfaces for TRAP, OC and RANKL (P>0.05), whereas OPG showed higher PEO labeling in the OVX group (P<0.05). Biomechanical analysis showed higher reverse torque values (N.cm) for PEO, irrespective of whether they were OVX or SHAM groups (P<0.05). Conclusion: The results indicated that the PEO texturing method favored bone formation and showed higher bone maturation levels during later periods in osteoporotic rats. (AU)

• Processo FAPESP: 16/20297-6 - A influência da texturização de superfície do Titânio comercialmente puro (Ticp) com incorporação de Ca e P produzida pela oxidação por plasma eletrolítico em ossos de baixa densidade: Um estudo in vitro, ex-vivo e in vivo
• Beneficiário: Leonardo Perez Faverani
• Modalidade de apoio: Auxílio à Pesquisa - Regular