| Texto completo | |
| Autor(es): Mostrar menos - |
Cavaleiro, Jessica
[1, 2]
;
Oliveira, Nathally B.
[1, 2]
;
Ribeiro, Talita A.
[1, 2]
;
Guimaraes, Lohaine F.
[1, 2]
;
Fernandes, Noemi M.
[3]
;
da Silva-Neto, Inacio D.
[3]
;
Marszaukowski, Flavia
[4]
;
Wohnrath, Karen
[4]
;
Barreto Jr, Cleber B.
;
Schweikert, Michael
[5]
;
Petroni, Giulio
[6]
;
Ortenzi, Claudio
[7]
;
Buonanno, Federico
[7]
;
Picciani, Paulo H. S.
[2, 8]
;
Oliveira Jr, Osvaldo N.
;
Soares, Carlos Augusto G.
[1, 2]
Número total de Autores: 16
|
| Afiliação do(s) autor(es): | [1] Univ Fed Rio de Janeiro, Inst Biol, Dept Genet, Rio De Janeiro - Brazil
[2] Univ Fed Rio de Janeiro, Biofunct Nanodevices Dev Grp, Rio De Janeiro - Brazil
[3] Univ Fed Rio de Janeiro, Dept Zool, Inst Biol, Rio De Janeiro - Brazil
[4] Univ Estadual Ponta Grossa, Dept Quim, Ponta Grossa, Parana - Brazil
[5] Univ Stuttgart, Dept Biobased Mat, Inst Biomat & Biomol Syst, Stuttgart - Germany
[6] Univ Pisa, Unita Protistol, Dipartimento Biol, Pisa - Italy
[7] Univ Macerate, Lab Protistol & Didatt Biol, Dipartimento SFBCT, UNIMC, Macerata - Italy
[8] Univ Fed Rio de Janeiro, Inst Macromol Eloise Mano, Rio De Janeiro - Brazil
Número total de Afiliações: 8
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Photochemistry and Photobiology; v. 96, n. 6 JUN 2020. |
| Citações Web of Science: | 1 |
| Resumo | |
Blepharismins are photodynamic hypericin-like dianthrones produced as a variable pigment blend in Blepharisma ciliates and mostly studied in the Afro-Asiatic Blepharisma japonicum. The present work describes the bioactivity of pigments from the Brazilian Blepharisma sinuosum. Comparative analyses showed that the pigments from both species can trigger photo-induced modifications in phospholipids, but different redox properties and biological activities were assigned for each pigment blend. Stronger activities were detected for B. sinuosum pigments, with the lethal concentration LC50 10 x lower than B. japonicum pigments in light-irradiated tests against Bacillus cereus and less than half for treatments on the human HeLa tumor cells. HPLC showed B. sinuosum producing a simpler pigment blend, mostly with the blepharismin-C (similar to 70%) and blepharismin-E (similar to 30%) types. Each blepharismin engaged a specific dose-response profile on sensitive cells. The blepharismin-B and blepharismin-C were the most toxic pigments, showing LC50 similar to 2.5-3.0 mu M and similar to 100 mu M on B. cereus and HeLa cells, respectively, after illumination. Similarity clustering analysis compiling the bioactivity data revealed two groups of blepharismins: the most active, B and C, and the less active, A, D and E. The B. sinuosum pigment blend includes one representative of each clade. Functional and medical implications are discussed. (AU) | |
| Processo FAPESP: | 13/14262-7 - Filmes nanoestruturados de materiais de interesse biológico |
| Beneficiário: | Osvaldo Novais de Oliveira Junior |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |