White, brown, and bone marrow adipose tissue behavior in DHEA-induced PCOS mice

Autor(es):Mostrar menos -

Doretto-Silva, Lorena ^[1] ; Steiner, Marcelo Luis ^[2] ; Veridiano, Juliana Mora ^[1] ; Petri, Giuliana ^[3] ; de Brito Luz, Maria Claudia ^[4] ; Neofiti-Papi, Bianca ^[5] ; Bianco, Bianca ^[6] ; Affonso Fonseca, Fernando Luiz ^[4] ; Szymanski de Toledo, Olga Maria ^[1] ; Fernandes, Cesar Eduardo ^[2] ; Pompei, Luciano de Melo ^[2]

Afiliação do(s) autor(es)

^[1] Ctr Univ Saude ABC, Dept Morphol & Physiol, Histol Lab, Santo Andre, SP - Brazil

^[2] Ctr Univ Saude ABC, Dept Gynecol & Obstet, Av Lauro Gomes 2000, BR-09060870 Santo Andre, SP - Brazil

^[3] Vivarium Ctr Univ Saude ABC, Santo Andre, SP - Brazil

^[4] Ctr Univ Saude ABC, Clin Anal Lab, Santo Andre, SP - Brazil

^[5] Univ Sao Paulo, Inst Biomed Sci 3, Dept Anat, Bone Metab Lab, Sao Paulo - Brazil

^[6] Ctr Univ Saude ABC, Lab Genet & Mol Biol, Santo Andre, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Gynecological Endocrinology; v. 37, n. 1 JUN 2020.
• Citações Web of Science: 0

Resumo

Introduction:To investigate the behavior of white, brown, and bone marrow adipose tissue (MAT) and the insulin resistance in a PCOS mice model. Methods:Thirty-one female C57BL/6J mice were divided into four groups: two were treated with subcutaneous dehydroepiandrosterone (DHEA) implants and divided into normal and hypercaloric diet (HFD). Two were control and divided into normal and HFD. Presence of insulin resistance, growth, and adipocyte markers expression of white and brown adipose tissues and growth and inflammatory cytokines expression of bone marrow adipose tissue were evaluated. Results:Hypercaloric diet groups presented higher total weight gain and huge growth in all fat sites, except bone marrow. They also demonstrated greater expression of adipocyte markers in sites of white adipose tissue. DHEA + HFD group showed more insulin intolerance than all other groups. DHEA shows to abrogate AdipoQexpression in all fatty tissues. Conclusions:DHEA alone does not influence adipose tissue growth, but contributes to increased insulin resistance and influences the expression of adipokines. Proximal MAT showed different behavior from the other fat depot. (AU)

• Processo FAPESP: 16/25244-8 - O impacto da síndrome dos ovários policísticos e da obesidade na expansão do tecido adiposo intramedular e na produção e secreção de adiponectina
• Beneficiário: Marcelo Luis Steiner
• Modalidade de apoio: Auxílio à Pesquisa - Regular