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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Chemical features of the photosensitizers new methylene blue N and S137 influence their subcellular localization and photoinactivation efficiency in Candida albicans

Texto completo
Autor(es):
Rodrigues, Gabriela Braga [1] ; Pereira Brancini, Guilherme Thomaz [1] ; Uyemura, Sergio Akira [1] ; Bachmann, Luciano [2] ; Wainwright, Mark [3] ; Leite Braga, Gilberto Ubida [1]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Dept Anal Clin Toxicol & Bromatol, Fac Ciencias Farmaceut Ribeirao Preto, BR-14040903 Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Fis, BR-14040903 Ribeirao Preto, SP - Brazil
[3] Liverpool John Moores Univ, Sch Pharm & Biomol Sci, Liverpool L3 3AF, Merseyside - England
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY; v. 209, AUG 2020.
Citações Web of Science: 1
Resumo

Antimicrobial photodynamic treatment (APDT) has emerged as an effective therapy against pathogenic fungi with both acquired and intrinsic resistance to commonly used antifungal agents. Success of APDT depends on the availability of effective photosensitizers capable of acting on different fungal structures and species. Among the phenothiazinium dyes tested as photoantifungals, new methylene blue N (NMBN) and the novel pentacyclic compound S137 are the most efficient. In the present study we compared the effects of APDT with NMBN and S137 on the survival of Candida albicans and employed a set of fluorescent probes (propidium iodide, FUN-1, JCS, DHR-123 and DHE) together with confocal microscopy and flow cytometry to evaluate the effects of these two chemically diverse photosensitizers on cell membrane permeability, metabolism and redox status, and mitochondrial activity. Taken together, our results indicate that, due to chemical features resulting in different lipophilicity, NMBN and S137 localize to distinct subcellular structures and hence inactivate C. albicans cells via different mechanisms. S137 localizes mostly to the cell membrane and, upon light exposure, photo-oxidizes membrane lipids. NMBN readily localizes to mitochondria and exerts its photodynamic effects there, which was observed to be a less effective way to achieve cell death at lower light fluences. (AU)

Processo FAPESP: 12/15204-8 - Estudo mecanístico da inativação fotodinâmica de fungos patógenos de humanos e de fungos fitopatógenos
Beneficiário:Gilberto Úbida Leite Braga
Modalidade de apoio: Auxílio à Pesquisa - Regular
Processo FAPESP: 12/22933-6 - Estudo dos mecanismos envolvidos na fotossensibilização de espécies de Candida e Trichophyton
Beneficiário:Gabriela Braga Rodrigues
Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 15/24305-0 - Avaliação dos efeitos da luz azul e da luz vermelha na tolerância ao estresse e no proteoma do fungo entomopatogênico Metarhizium acridum
Beneficiário:Guilherme Thomaz Pereira Brancini
Modalidade de apoio: Bolsas no Brasil - Doutorado