Nanosensors based on LSPR are able to serologically differentiate dengue from Zika infections

Autor(es)

Versiani, Alice F. ^{[1, 2, 3]} ; Martins, Estefania M. N. ^{[2, 4]} ; Andrade, Lidia M. ^{[5, 2]} ; Cox, Laura ^[1] ; Pereira, Glauco C. ^[6] ; Barbosa-Stancioli, Edel F. ^[1] ; Nogueira, Mauricio L. ^[7] ; Ladeira, Luiz O. ^{[5, 2]} ; da Fonseca, Flavio G. ^{[1, 8]}

Afiliação do(s) autor(es)

^[1] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Microbiol, Lab Virol Basica & Aplicada, Belo Horizonte, MG - Brazil

^[2] Univ Fed Minas Gerais, Dept Fis, NanoBioMed Res Grp, Belo Horizonte, MG - Brazil

^[3] Fac Med Sao Jose Rio Do Preto, Dept Doencas Infecciosas & Parasitarias, Lab Pesquisa Virol, Sao Jose Do Rio Preto, SP - Brazil

^[4] Ctr Desenvolvimento Tecnol Nucl CDTN CNEN, Lab Quim Nanoestruturas Carbono, Belo Horizonte, MG - Brazil

^[5] Univ Fed Minas Gerais, Dept Fis, Lab Nanomat, Belo Horizonte, MG - Brazil

^[6] Fundacao Ezequiel Dias, FUNED, Belo Horizonte, MG - Brazil

^[7] Fac Med Sao Jose Do Rio Preto, Dept Doencas Infecciosas & Parasitarias, Lab Pesquisa Virol, Sao Jose Do Rio Preto, SP - Brazil

^[8] Univ Fed Minas Gerais, Ctr Tecnol Vacinas, Belo Horizonte, MG - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 10, n. 1 JUL 9 2020.
• Citações Web of Science: 4

Resumo

The Flaviviridae virus family was named after the Yellow-fever virus, and the latin term flavi means ``of golden color{''}. Dengue, caused by Dengue virus (DENV), is one of the most important infectious diseases worldwide. A sensitive and differential diagnosis is crucial for patient management, especially due to the occurrence of serological cross-reactivity to other co-circulating flaviviruses. This became particularly important with the emergence of Zika virus (ZIKV) in areas were DENV seroprevalence was already high. We developed a sensitive and specific diagnostic test based on gold nanorods (GNR) functionalized with DENV proteins as nanosensors. These were able to detect as little as one picogram of anti-DENV monoclonal antibodies and highly diluted DENV-positive human sera. The nanosensors could differentiate DENV-positive sera from other flavivirus-infected patients, including ZIKV, and were even able to distinguish which DENV serotype infected individual patients. Readouts are obtained in ELISA-plate spectrophotometers without the need of specific devices. (AU)

• Processo FAPESP: 13/21719-3 - Estudo epidemiológico da dengue (sorotipos 1 a 4) em coorte prospectiva de São José do Rio Preto, São Paulo, Brasil, durante 2014 a 2018
• Beneficiário: Maurício Lacerda Nogueira
• Modalidade de apoio: Auxílio à Pesquisa - Temático