| Full text | |
| Author(s): |
Versiani, Alice F.
[1, 2, 3]
;
Martins, Estefania M. N.
[2, 4]
;
Andrade, Lidia M.
[5, 2]
;
Cox, Laura
[1]
;
Pereira, Glauco C.
[6]
;
Barbosa-Stancioli, Edel F.
[1]
;
Nogueira, Mauricio L.
[7]
;
Ladeira, Luiz O.
[5, 2]
;
da Fonseca, Flavio G.
[1, 8]
Total Authors: 9
|
| Affiliation: | [1] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Microbiol, Lab Virol Basica & Aplicada, Belo Horizonte, MG - Brazil
[2] Univ Fed Minas Gerais, Dept Fis, NanoBioMed Res Grp, Belo Horizonte, MG - Brazil
[3] Fac Med Sao Jose Rio Do Preto, Dept Doencas Infecciosas & Parasitarias, Lab Pesquisa Virol, Sao Jose Do Rio Preto, SP - Brazil
[4] Ctr Desenvolvimento Tecnol Nucl CDTN CNEN, Lab Quim Nanoestruturas Carbono, Belo Horizonte, MG - Brazil
[5] Univ Fed Minas Gerais, Dept Fis, Lab Nanomat, Belo Horizonte, MG - Brazil
[6] Fundacao Ezequiel Dias, FUNED, Belo Horizonte, MG - Brazil
[7] Fac Med Sao Jose Do Rio Preto, Dept Doencas Infecciosas & Parasitarias, Lab Pesquisa Virol, Sao Jose Do Rio Preto, SP - Brazil
[8] Univ Fed Minas Gerais, Ctr Tecnol Vacinas, Belo Horizonte, MG - Brazil
Total Affiliations: 8
|
| Document type: | Journal article |
| Source: | SCIENTIFIC REPORTS; v. 10, n. 1 JUL 9 2020. |
| Web of Science Citations: | 4 |
| Abstract | |
The Flaviviridae virus family was named after the Yellow-fever virus, and the latin term flavi means ``of golden color{''}. Dengue, caused by Dengue virus (DENV), is one of the most important infectious diseases worldwide. A sensitive and differential diagnosis is crucial for patient management, especially due to the occurrence of serological cross-reactivity to other co-circulating flaviviruses. This became particularly important with the emergence of Zika virus (ZIKV) in areas were DENV seroprevalence was already high. We developed a sensitive and specific diagnostic test based on gold nanorods (GNR) functionalized with DENV proteins as nanosensors. These were able to detect as little as one picogram of anti-DENV monoclonal antibodies and highly diluted DENV-positive human sera. The nanosensors could differentiate DENV-positive sera from other flavivirus-infected patients, including ZIKV, and were even able to distinguish which DENV serotype infected individual patients. Readouts are obtained in ELISA-plate spectrophotometers without the need of specific devices. (AU) | |
| FAPESP's process: | 13/21719-3 - Epidemiological study of dengue (serotypes1-4) in a cohort of São José do Rio Preto, São Paulo, Brazil, during 2014-2018 |
| Grantee: | Maurício Lacerda Nogueira |
| Support Opportunities: | Research Projects - Thematic Grants |