Sex Chromosomes and Internal Telomeric Sequences inDormitator latifrons(Richardson 1844) (Eleotridae: Eleotrinae): An Insight into Their Origin in the Genus

Autor(es)

Paim, Fabilene Gomes ^[1] ; Nirchio, Mauro ^{[2, 3]} ; Oliveira, Claudio ^[1] ; Rossi, Anna Rita ^[4]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista UNESP, Dept Biol Estrutural & Func, Inst Biociencias, BR-18618689 Botucatu, SP - Brazil

^[2] Univ Tecn Machala, Av Panamer Km 5 1-2, Via Pasaje, Machala 070151, El Oro - Ecuador

^[3] Univ Oriente, Nucleo Nueva Esparta, Escuela Ciencias Aplicadas Mar, Porlamar 6301, Isla Margarita - Venezuela

^[4] Sapienza Univ Roma, Dipartimento Biol & Biotecnol C Darwin, Via Alfonso Borelli 50, I-00161 Rome - Italy

• Tipo de documento: Artigo Científico
• Fonte: GENES; v. 11, n. 6 JUN 2020.
• Citações Web of Science: 1

Resumo

The freshwater fish speciesDormitator latifrons, commonly named the Pacific fat sleeper, is an important food resource in CentralSouth America, yet almost no genetic information on it is available. A cytogenetic analysis of this species was undertaken by standard and molecular techniques (chromosomal mapping of 18S rDNA, 5S rDNA, and telomeric repeats), aiming to describe the karyotype features, verify the presence of sex chromosomes described in congeneric species, and make inferences on chromosome evolution in the genus. The karyotype (2n = 46) is mainly composed of metacentric and submetacentic chromosomes, with nucleolar organizer regions (NORs) localized on the short arms of submetacentric pair 10. The presence of XX/XY sex chromosomes was observed, with the X chromosome carrying the 5S rDNA sequences. These heterochromosomes likely appeared before 1 million years ago, since they are shared with another derivedDormitatorspecies (Dormitator maculatus) distributed in the Western Atlantic. Telomeric repeats hybridize to the terminal portions of almost all chromosomes; additional interstitial sites are present in the centromeric region, suggesting pericentromeric inversions as the main rearrangement mechanisms that has driven karyotypic evolution in the genus. The data provided here contribute to improving the cytogenetics knowledge ofD. latifrons, offering basic information that could be useful in aquaculture farming of this neotropical fish. (AU)

• Processo FAPESP: 14/26508-3 - Filogenia da hiperdiversa ordem Characiformes (Teleostei: Ostariophysi) utilizando elementos ultraconservados
• Beneficiário: Claudio de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 18/20610-1 - Estudo da biogeografia da costa do Brasil utilizando dados da genética de populações de bagres marinhos dos gêneros Cathorops e Genidens
• Beneficiário: Claudio de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/09204-6 - Biodiversidade e Biogeografia em peixes marinho-estuarinos (Teleostei) do Brasil
• Beneficiário: Claudio de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular