Rotary-jet spun polycaprolactone/nano-hydroxyapatite scaffolds modified by simulated body fluid influenced the flexural mode of the neoformed bone

Autor(es)

Vasconcellos, Luana M. R. ^[1] ; Elias, Conceicao de M. V. ^[2] ; Minhoto, Giovanna B. ^[1] ; Abdala, Julia M. A. ^[2] ; Andrade, Telmo M. ^{[2, 3]} ; de Araujo, Juliani C. R. ^[1] ; Gusmao, Suziete Batista Soares ^[4] ; Viana, Bartolomeu C. ^{[5, 4]} ; Marciano, Fernanda R. ^[5] ; Lobo, Anderson O. ^[4]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, Inst Sci & Technol, Dept Biosci & Oral Diag, BR-12245000 Sao Jose Dos Campos, SP - Brazil

^[2] Univ Brasil, Inst Cient & Tecnol, BR-08230030 Sao Paulo - Brazil

^[3] Uninassau Univ, BR-64017775 Teresina, Piaui - Brazil

^[4] UFPI Fed Univ Piaui, Dept Phys, BR-64017775 Teresina, Piaui - Brazil

^[5] UFPI Fed Univ Piaui, Dept Mat Engn, LIMAV Interdisciplinary Lab Adv Mat, BioMatLab, BR-64049550 Teresina, Piaui - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE; v. 31, n. 8 JUL 27 2020.
• Citações Web of Science: 0

Resumo

Polycaprolactone (PCL) is a biocompatible, biodegradable synthetic polymer which in combination with nanohydroxyapatite (nHAp) can give rise to a low cost, nontoxic bioactive product with excellent mechanical properties and slow degradation. Here we produced, characterized and evaluated in vivo the bone formation of PCL/nHAp scaffolds produced by the rotary jet spinning technique. The scaffolds produced were firstly soaked into simulated body fluid for 21 days to also obtain nHAp onto PCL/nHAp scaffolds. Afterwards, the scaffolds were characterized by scanning electron microscopy (SEM), energy dispersive spectroscopy and Raman spectroscopy. For in vivo experiments, 20 male Wistar rats were used and randomly divided in 4 experimental groups (n = 5). A critical defect of 3 mm in diameter was made in the tibia of the animals, which were filled with G1 control (clot); G2-PCL scaffold; G3-PCL/nHAp (5%) scaffold; G4-PCL/nHAp (20%) scaffold. All animals were euthanized 60 days after surgery, and the bone repair in the right tibiae were evaluated by radiographic analysis, histological analysis and histomorphometric analysis. While in the left tibias, the areas of bone repair were submitted to the flexural strength test. Radiographic and histomorphometric analyses no showed statistical difference in new bone formation between the groups, but in the three-point flexural tests, the PCL/nHAp (20%) scaffold positively influenced the flexural mode of the neoformed bone. These findings indicate that PCL/nHAp (20%) scaffold improve biomechanical properties of neoformed bone and could be used for bone medicine regenerative. (AU)

• Processo FAPESP: 16/20820-0 - Análise da influência de scaffolds de policaprolactona (PCL) associados com nanohidroxiapatita (nHA), produzidos por rotofiação, no reparo ósseo
• Beneficiário: Giovanna Bignoto Minhoto
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica