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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Fungistatic Action of N-Acetylcysteine onCandida albicansBiofilms and Its Interaction with Antifungal Agents

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Autor(es):
Bezerra Santos Nunes, Thais Soares [1] ; Rosa, Leticia Matheus [1] ; Vega-Chacon, Yuliana [1] ; de Oliveira Mima, Ewerton Garcia [1]
Número total de Autores: 4
Afiliação do(s) autor(es):
[1] Sao Paulo State Univ UNESP, Sch Dent, Dept Dent Mat & Prosthodont, Lab Appl Microbiol, BR-14801903 Araraquara, SP - Brazil
Número total de Afiliações: 1
Tipo de documento: Artigo Científico
Fonte: MICROORGANISMS; v. 8, n. 7 JUL 2020.
Citações Web of Science: 0
Resumo

Therapies targeted to fungal biofilms, mainly against the matrix, and therapies that do not induce microbial resistance are relevant. N-acetylcysteine (NAC), a mucolytic agent, has shown antimicrobial action. This study evaluated the effect of NAC against fluconazole-susceptible (CaS) and -resistant (CaR)Candida albicans. The susceptibility of planktonic cultures to NAC, the effect of NAC on biofilms and their matrix, the interaction of NAC with antifungal agents, and confocal microscopy were evaluated. Data were analyzed descriptively and by the ANOVA/Welch and Tukey/Gomes-Howell tests. The minimum inhibitory concentration (MIC) of NAC was 25 mg/mL for both strains. NAC significantly reduced the viability of both fungal strains. Concentrations higher than the MIC (100 and 50 mg/mL) reduced the viability and the biomass. NAC at 12.5 mg/mL increased the fungal viability. NAC also reduced the soluble components of the biofilm matrix, and showed synergism with caspofungin against planktonic cultures of CaS, but not against biofilms. Confocal images demonstrated that NAC reduced the biofilm thickness and the fluorescence intensity of most fluorochromes used. High concentrations of NAC had similar fungistatic effects against both strains, while a low concentration showed the opposite result. The antibiofilm action of NAC was due to its fungistatic action. (AU)

Processo FAPESP: 18/02513-9 - Quantificação de Candida albicans, efeito de inibidores de bomba de efluxo, da N-acetilcisteína e de micelas fotoativadas e validação de um modelo in vivo de estomatite protética
Beneficiário:Ewerton Garcia de Oliveira Mima
Modalidade de apoio: Auxílio à Pesquisa - Regular