Leucine-Rich Diet Modulates the Metabolomic and Proteomic Profile of Skeletal Muscle during Cancer Cachexia

Autor(es):Mostrar menos -

Cruz, Bread ^[1] ; Oliveira, Andre ^[1] ; Viana, Lais Rosa ^[1] ; Lopes-Aguiar, Leisa ^[1] ; Canevarolo, Rafael ^[2] ; Colombera, Maiara Caroline ^[1] ; Valentim, Rafael Rossi ^[1] ; Garcia-Fossa, Fernanda ^[3] ; de Sousa, Lizandra Maia ^[4] ; Castelucci, Bianca Gazieri ^[4] ; Consonni, Silvio Roberto ^[4] ; Martins-de-Souza, Daniel ^{[5, 6, 7]} ; de Jesus, Marcelo Bispo ^[3] ; Russell, Steven Thomas ^[8] ; Cintra Gomes-Mardondes, Maria Cristina ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Dept Struct & Funct Biol, Inst Biol, BR-13083862 Campinas, SP - Brazil

^[2] H Lee Moffitt Canc Ctr & Res Inst, Dept Canc Physiol, Tampa, FL 33612 - USA

^[3] Univ Estadual Campinas, Dept Biochem & Tissue Biol, Nanocell Interact Lab, Inst Biol, BR-13083862 Campinas, SP - Brazil

^[4] Univ Estadual Campinas, Dept Biochem & Tissue Biol, Inst Biol, BR-13083862 Campinas, SP - Brazil

^[5] Univ Estadual Campinas, Inst Biol, Dept Biochem & Tissue Biol, Lab Neuroprote, BR-13083862 Campinas, SP - Brazil

^[6] Univ Estadual Campinas, Expt Med Res Cluster EMRC, BR-13083862 Campinas, SP - Brazil

^[7] DOr Inst Res & Educ IDOR, BR-04501000 Sao Paulo - Brazil

^[8] Aston Univ, Coll Hlth & Life Sci, Sch Biosci, Birmingham B4 7ET, W Midlands - England

• Tipo de documento: Artigo Científico
• Fonte: CANCERS; v. 12, n. 7 JUL 2020.
• Citações Web of Science: 0

Resumo

Background:Cancer-cachexia induces a variety of metabolic disorders, including skeletal muscle imbalance. Alternative therapy, as nutritional supplementation with leucine, shows a modulatory effect over tumour damage in vivo and in vitro.Method:Adult rats distributed into Control (C), Walker tumour-bearing (W), control fed a leucine-rich diet (L), and tumour-bearing fed a leucine-rich diet (WL) groups had the gastrocnemius muscle metabolomic and proteomic assays performed in parallel to in vitro assays.Results:W group presented an affected muscle metabolomic and proteomic profile mainly related to energy generation and carbohydrates catabolic processes, but leucine-supplemented group (WL) recovered the energy production. In vitro assay showed that cell proliferation, mitochondria number and oxygen consumption were higher under leucine effect than the tumour influence. Muscle proteomics results showed that the main affected cell component was mitochondria, leading to an impacted energy generation, including impairment in proteins of the tricarboxylic cycle and carbohydrates catabolic processes, which were modulated and improved by leucine treatment.Conclusion: In summary, we showed a beneficial effect of leucine upon mitochondria, providing information about the muscle glycolytic pathways used by this amino acid, where it can be associated with the preservation of morphometric parameters and consequent protection against the effects of cachexia. (AU)

• Processo FAPESP: 15/06766-0 - Nutrição e câncer: avaliação do perfil metabolômico, proteômico e de vias de sinalização celular em músculo esquelético e tecido tumoral sob influência da suplementação de leucina durante o desenvolvimento in vitro e in vivo do Tumor de Walker
• Beneficiário: Bread Leandro Gomes da Cruz
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 17/02739-4 - Nutrição e câncer: estudo dos aspectos moleculares, proteômicos e metabolômicos em modelo experimental de caquexia
• Beneficiário: Maria Cristina Cintra Gomes Marcondes
• Modalidade de apoio: Auxílio à Pesquisa - Temático