| Texto completo | |
| Autor(es): Mostrar menos - |
da Fonseca Junior, Aldcejam Martins
[1]
;
Gaita, Vincenzo
[2]
;
Argumedo, Daniel Ricardo
[1]
;
de Castro, Leticia Signori
[3]
;
de Agostini Losano, Joao Diego
[3]
;
Leite, Roberta Ferreira
[3]
;
Nichi, Marcilio
[3]
;
d'Avila Assumpcao, Mayra Elena Ortiz
[3]
;
de Araujo, Daniele Ribeiro
[1]
;
Ranha Neves, Antonio Alvaro
[1]
;
Milazzotto, Marcella Pecora
[1]
Número total de Autores: 11
|
| Afiliação do(s) autor(es): | [1] Fed Univ ABC, Ctr Nat & Human Sci, Av Estados 5001, BR-09210580 Santo Andre, SP - Brazil
[2] Univ Napoli Federico II, Dipartimento Med Vet & Prod Anim, Naples - Italy
[3] Univ Sao Paulo, Fac Vet Med & Anim Sci, Sao Paulo - Brazil
Número total de Afiliações: 3
|
| Tipo de documento: | Artigo Científico |
| Fonte: | REPRODUCTION IN DOMESTIC ANIMALS; v. 55, n. 8, p. 974-983, AUG 2020. |
| Citações Web of Science: | 0 |
| Resumo | |
The female reproductive tract, in particular the composition of the uterine and oviduct fluids, is responsible, at least in part, for triggering sperm cell modifications, essential for the acquisition of fertilization ability. Hyaluronic acid (HA) is a glycosaminoglycan present in these fluids, and its role in the fertilization process and sperm functionality is still barely understood. This work was designed to (a) determine the rheological characteristics of the fertilization medium by the addition of HA and (b) determine the HA influence on sperm motility and functional status. To that end, the in vitro fertilization medium was supplemented with 4 doses of HA (6, 60, 600 and 6,000 mu g/ml) and analysed for viscosity and adhesion strength characteristics. Then, thawed semen from 6 bulls were incubated in these media and assessed at 4 different moments for morphological and functional parameters (plasma and acrosomal membrane integrities, mitochondrial membrane potential, capacitation, acrosomal reaction, and motility). The rheological evaluation showed that the addition of HA was able to increase both the viscosity and the adhesion strength of the fertilization medium, especially in the 6,000 mu g/ml group in which the effect was more pronounced. No influence of HA could be observed on mitochondrial potential, and acrosomal and plasma membrane integrities. However, HA supplementation, at lower doses, led to an increase in the number of reacted sperm, as well as changes in motility parameters, with increase in the number of motile, rapid and progressive spermatozoa. In conclusion, the addition of HA alters the rheological properties of the fertilization medium and leads to the improvement of the properties related to sperm motility and capacitation, without compromising other functional aspects of the cell. (AU) | |
| Processo FAPESP: | 17/18384-0 - Metaboloepigenética: o estudo do desenvolvimento de embriões bovinos sob uma nova ótica |
| Beneficiário: | Marcella Pecora Milazzotto |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |