Plasma membrane H+-ATPases sustain pollen tube growth and fertilization

Autor(es):Mostrar menos -

Hoffmann, Robert D. ^[1] ; Portes, Maria Teresa ^[2] ; Olsen, Lene Irene ^[1] ; Damineli, Daniel Santa Cruz ^{[2, 3]} ; Hayashi, Maki ^[1] ; Nunes, Custodio O. ^[2] ; Pedersen, Jesper T. ^[1] ; Lima, Pedro T. ^[4] ; Campos, Claudia ^[4] ; Feijo, Jose A. ^{[2, 4]} ; Palmgren, Michael ^[1]

Afiliação do(s) autor(es)

^[1] Univ Copenhagen, Dept Plant & Environm Sci, DK-1871 Frederiksberg C - Denmark

^[2] Univ Maryland, Dept Cell Biol & Mol Genet, College Pk, MD 20742 - USA

^[3] Univ Sao Paulo, Dept Pediat, Fac Med, BR-01246903 Sao Paulo, SP - Brazil

^[4] Ist Gulbenkian Ciencia, P-2780156 Oeiras - Portugal

• Tipo de documento: Artigo Científico
• Fonte: NATURE COMMUNICATIONS; v. 11, n. 1 MAY 14 2020.
• Citações Web of Science: 1

Resumo

Pollen tubes are highly polarized tip-growing cells that depend on cytosolic pH gradients for signaling and growth. Autoinhibited plasma membrane proton (H+) ATPases (AHAs) have been proposed to energize pollen tube growth and underlie cell polarity, however, mechanistic evidence for this is lacking. Here we report that the combined loss of AHA6, AHA8, and AHA9 in Arabidopsis thaliana delays pollen germination and causes pollen tube growth defects, leading to drastically reduced fertility. Pollen tubes of aha mutants had reduced extracellular proton (H+) and anion fluxes, reduced cytosolic pH, reduced tip-to-shank proton gradients, and defects in actin organization. Furthermore, mutant pollen tubes had less negative membrane potentials, substantiating a mechanistic role for AHAs in pollen tube growth through plasma membrane hyperpolarization. Our findings define AHAs as energy transducers that sustain the ionic circuit defining the spatial and temporal profiles of cytosolic pH, thereby controlling downstream pH-dependent mechanisms essential for pollen tube elongation, and thus plant fertility. Cytosolic ion gradients in growing pollen tubes are thought to be required for polar growth. Here the authors show that the Arabidopsis plasma membrane H+ ATPases, AHA6, AHA8, and AHA9, maintain tip-to-shank proton gradients, oscillations in cytosolic pH and actin organization to enable pollen tube elongation. (AU)

• Processo FAPESP: 19/23343-7 - Análise temporal da expressão gênica
• Beneficiário: Daniel Santa Cruz Damineli
• Linha de fomento: Bolsas no Brasil - Pós-Doutorado