Simvastatin-Enriched Macro-Porous Chitosan-Calcium-Aluminate Scaffold for Mineralized Tissue Regeneration

Autor(es)

Fernanda Balestrero Cassiano ^[1] ; Diana Gabriela Soares ^[2] ; Ester Alves Ferreira Bordini ^[3] ; Giovana Anovazzi ^[4] ; Josimeri Hebling ^[5] ; Carlos Alberto de Souza Costa ^[6]

Afiliação do(s) autor(es)

^[1] Universidade Estadual Paulista. Araraquara School of Dentistry. Department of Physiology and Pathology - Brasil

^[2] Universidade de São Paulo. Bauru School of Dentistry. Department of Operative Dentistry, Endodontics and Dental Materials - Brasil

^[3] Universidade Estadual Paulista. Araraquara School of Dentistry. Department of Physiology and Pathology - Brasil

^[4] Universidade Estadual Paulista. Araraquara School of Dentistry. Department of Orthodontics and Pediatric Dentistry - Brasil

^[5] Universidade Estadual Paulista. Araraquara School of Dentistry. Department of Orthodontics and Pediatric Dentistry - Brasil

^[6] Universidade Estadual Paulista. Araraquara School of Dentistry. Department of Physiology and Pathology - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Dental Journal; v. 31, n. 4, p. 385-391, 2020-09-04.

Resumo

Abstract The present study evaluated the odontogenic potential of human dental pulp cells (HDPCs) exposed to chitosan scaffolds containing calcium aluminate (CHAlCa) associated or not with low doses of simvastatin (SV). Chitosan scaffolds received a suspension of calcium aluminate (AlCa) and were then immersed into solutions containing SV. The following groups were established: chitosan-calcium-aluminate scaffolds (CHAlCa - Control), chitosan calcium-aluminate with 0.5 µM SV (CHAlCa-SV0.5), and chitosan calcium-aluminate with 1.0 µM SV (CHAlCa-SV1.0). The morphology and composition of the scaffolds were evaluated by SEM and EDS, respectively. After 14 days of HDPCs culture on scaffolds, cell viability, adhesion and spread, mineralized matrix deposition as well as gene expression of odontogenic markers were assessed. Calcium aluminate particles were incorporated into the chitosan matrix, which exhibited regular pores homogeneously distributed throughout its structure. The selected SV dosages were biocompatible with HDPCs. Chitosan-calcium-aluminate scaffolds with 1 µM SV induced the odontoblastic phenotype in the HDPCs, which showed enhanced mineralized matrix deposition and up-regulated ALP, Col1A1, and DMP-1 expression. Therefore, one can conclude that the incorporation of calcium aluminate and simvastatin in chitosan scaffolds had a synergistic effect on HDPCs, favoring odontogenic cell differentiation and mineralized matrix deposition. (AU)

• Processo FAPESP: 16/06704-8 - Desenvolvimento e análise biológica de um scaffold macro-poroso de quitosana-cálcio para regeneração do complexo dentino-pulpar
• Beneficiário: Fernanda Balestrero Cassiano
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 17/20181-0 - Engenharia Tecidual e Biotecnologia aplicadas no desenvolvimento de scaffolds macroporosos contendo cálcio e com nano-topografia de superfície para modulação da regeneração do complexo dentino-pulpar
• Beneficiário: Ester Alves Ferreira Bordini Galvani
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 16/15674-5 - Associação de técnicas de engenharia tecidual para modulação da regeneração de tecidos mineralizados sob inflamação degenerativa: análise em modelos de cultura-3D em biorreator de perfusão e inflamatórios em animais
• Beneficiário: Diana Gabriela Soares dos Passos
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores