Remodeling process in bone of aged rats in response to resistance training

Autor(es):Mostrar menos -

de Farias Junior, Goncalo Carreiro ^{[1, 2]} ; Neto, Ivo Vieira de Sousa ^[2] ; Guzzoni, Vinicius ^[3] ; Pisani, Graziele Deriggi ^[4] ; Royer, Carine ^[5] ; de Lima, Caroline Lourenco ^[5] ; Neves, Francisco de Assis Rocha ^[5] ; Bogni, Fabio Henrique ^[4] ; Nonaka, Keico Okino ^[4] ; Durigan, Joao Luiz Quagliotti ^{[1, 2]} ; Selistre-de-Araujo, Heloisa Sobreiro ^[4] ; Marqueti, Rita de Cassia ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Brasilia, Grad Program Rehabil Sci, Brasilia, DF - Brazil

^[2] Univ Brasilia, Fac Ceilandia, Lab Mol Anal, Brasilia, DF - Brazil

^[3] Univ Fed Paraiba, Dept Cellular & Mol Biol, Joao Pessoa, Paraiba - Brazil

^[4] Univ Fed Sao Carlos, Dept Physiol Sci, Sao Paulo - Brazil

^[5] Univ Brasilia, Fac Hlth Sci, Lab Mol Pharmacol, Brasilia, DF - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Life Sciences; v. 256, SEP 1 2020.
• Citações Web of Science: 0

Resumo

Aims: We investigate the effects of RT on the mechanical function, gene, and protein expression of key factors involved in bone remodeling during aging. Main methods: Male rats of 3 and 21 months of age were randomly allocated into four groups (8 per group): young sedentary (YS), young trained (YT), old sedentary (OS), and old trained (OT). RT was performed three times per week (12 weeks). Bone tenacity and stiffness were measured by biomechanical tests and mRNA levels of COL1A1, MEPE, SOST, OPG, BMP-2, PPAR-y, MMP-2-9-13, and TIMP-1 were evaluated by quantitative PCR. COL1A1 protein and MMP-2 activity were detected by western blotting and zymography assays. Key findings: Aging increased stiffness, while BMP-2, OPG, COL1A1 and MMP-2 mRNA levels reduced (OS vs YS; p <= 0.05). RT increased the tenacity of the femur and reduced PPAR-gamma regardless of age (YT vs. YS; OT vs. OS; p <= 0.05). RT downregulated SOST mRNA levels only in the OT group (vs. OS group, p <= 0.05). RT mitigated the age-associated increase in MMP-9 mRNA levels (p <= 0.05). In young animals, upregulation in MEPE, MMP-13, TIMP-1 were observed after RT, as well an increase in COL1A1 protein and MMP-2 activity (p <= 0.05). Significance: RT improved bone tenacity independent of aging, which is relevant for mechanical function, while, at protein levels, RT upregulated MMP-2 activity and collagen 1 only in young rats. This study highlights the importance of exercise on bone health and identifies specific molecular changes in response to RT. Our findings provide insights into the mechanisms involved in age-related changes. (AU)

• Processo FAPESP: 10/19390-5 - Matriz extracelular no envelhecimento e suas adaptações ao treinamento de força no tendão calcâneo de ratos: abordagem molecular, celular e biomecânica.
• Beneficiário: Rita de Cássia Marqueti Durigan
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 11/11229-3 - Matriz extracelular no envelhecimento e suas adaptações ao treinamento de força no tendão calcâneo de ratos: abordagem molecular, celular e biomecânica
• Beneficiário: Heloisa Sobreiro Selistre de Araújo
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/00798-2 - A matriz extracelular no envelhecimento, no exercício e no microambiente tumoral
• Beneficiário: Heloisa Sobreiro Selistre de Araújo
• Modalidade de apoio: Auxílio à Pesquisa - Temático