p53 and p21 expression in bone marrow clots of megaloblastic anemia patients

Autor(es)

Kwan, Denis Nicolas ^[1] ; Queiroz Rocha, Julia Thalita ^{[2, 3]} ; Niero-Melo, Ligia ^[4] ; Custodio Domingues, Maria Aparecida ^[2] ; Oliveira, Cristiano Claudino ^{[5, 6]}

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ FMB UNESP, Botucatu Med Sch, Botucatu, SP - Brazil

^[2] Sao Paulo State Univ FMB UNESP, Botucatu Med Sch, Dept Pathol, Botucatu, SP - Brazil

^[3] Sao Paulo State Univ FMB UNESP, Botucatu Med Sch, Salomao & Zoppi DASA, Botucatu, SP - Brazil

^[4] Sao Paulo State Univ FMB UNESP, Botucatu Med Sch, Dept Clin Med, Botucatu, SP - Brazil

^[5] Sao Paulo State Univ FMB UNESP, Botucatu Med Sch, Dept Pathol, 266 Bairro Jardim Oriental, BR-04321120 Sao Paulo, SP - Brazil

^[6] Hosp Sao Luiz D, Dept Anat Pathol, 266 Bairro Jardim Oriental, BR-04321120 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY; v. 13, n. 7, p. 1829-1833, 2020.
• Citações Web of Science: 0

Resumo

` The pathogenesis of megaloblastic hemopathies (MH) is centered on the deficiency of vitamin B12 and folic acid with interruption of erythrocyte maturation. This study researched the participation of p53 and p21 in the pathophysiology of the disease. A retrospective study enrolled 95 patients with histopathologic diagnosis by biopsy or bone marrow clot (BMB/BMC), with clinical review and immunohistochemical study in tissue microarray (TMA) for p53 and p21, detailing their marking location. All patients had BMC and only 11 had BMB. The CMO was a differential of this study and it allowed an expanded sample. In the TMA, 63.7% (58/91) of the samples were immunopositive for p53; and 35.2% (31/88) were immunopositive for p21. Nuclear staining, divergent from the literature, was observed in 17.3% (10/58) among those p53+ and in 38.7% (12/31) among those p21+. The pattern of immunostaining showed non-significant differences (P=0.474) regarding morphologic and clinical aspects. The positivity for both may indicate an effective balance between apoptosis and anti-apoptotic action. Excessive inhibition of apoptosis would contribute to high global cellularity, but without functional maturation effectiveness. In conclusion, there is p21 and/or p53 immunoexpression in most cases of this study and there is no clear association between immunoexpression pattern and patient outcome. Unlike the literature, we also found a percentage of nuclear immunostaining, but the finding was not statistically significant. Combination of p21 and p53 results created different possibilities of pathologic interpretation for MH, reinforcing the importance of studies similar to this one. (AU)

• Processo FAPESP: 16/19725-3 - Estudo da expressão de p53 e p21 em amostras de coágulo de medula óssea de pacientes com anemia megaloblástica
• Beneficiário: Denis Nicolas Kwan
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica