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Effects of 17 beta-estradiol on early gonadal development and expression of genes implicated in sexual differentiation of a South American teleost, Astyanax altiparanae

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Autor(es):
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Martinez-Bengochea, A. [1] ; Doretto, L. [1] ; Rosa, I. F. [1] ; Oliveira, M. A. [1] ; Silva, C. [1] ; Silva, D. M. Z. A. [1] ; Santos, G. R. [2, 3] ; Santos, J. S. F. [2, 3] ; Avelar, M. M. [2, 3] ; Silva, V, L. ; Lucianelli-Junior, Jr., D. [4] ; Souza, E. R. B. [5, 6] ; Silva, R. C. [5, 6] ; Stewart, A. B. [7] ; Nakaghi, L. S. O. [5, 6] ; Valentin, F. N. [4] ; Nobrega, R. H. [1]
Número total de Autores: 17
Afiliação do(s) autor(es):
[1] Sao Paulo State Univ, Inst Biosci, Dept Struct & Funct Biol, Reprod & Mol Biol Grp, Botucatu, SP - Brazil
[2] Sao Paulo State Univ, Fac Agr & Vet Sci, Dept Anim Morphol & Physiol, Jaboticabal, SP - Brazil
[3] Sao Paulo State Univ, Aquaculture Ctr CAUNESP, Jaboticabal, SP - Brazil
[4] Univ Fed Para, UFPA, Fac Med, Lab Morfofisiol, Altamira, Para - Brazil
[5] Silva, L., V, Sao Paulo State Univ, Fac Agr & Vet Sci, Dept Anim Morphol & Physiol, Jaboticabal, SP - Brazil
[6] Silva, L., V, Sao Paulo State Univ, Aquaculture Ctr CAUNESP, Jaboticabal, SP - Brazil
[7] West Virginia Univ, Dept Orthopaed Musculoskeletal Res, Morgantown, WV 26506 - USA
Número total de Afiliações: 7
Tipo de documento: Artigo Científico
Fonte: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY; v. 248, OCT-NOV 2020.
Citações Web of Science: 0
Resumo

Gonadal sex differentiation in teleost fish shows greater plasticity as compared to other vertebrates, as it can be influenced by a variety of factors such as exogenous sex steroids. Exogenous estrogens, such as 17 beta-estradiol (E2), can induce feminization when administered during early embryonic development. However, the mechanisms underlying the E2-induced feminization are not fully understood, especially in Neotropical species. Therefore, the aim of this study was to evaluate the effects of E2 administration on the phenotypic sex characteristics, histological assessment of the gonads, and the expression of selected genes in Astyanax altiparanae exposed to dietary E2 prior to gonadal differentiation. M 4 days post-hatch (dph), groups of 30-40 undifferentiated larvae were fed with a diet containing varying amounts of E2 for 28 days, and fish were sampled at 90 dph. Previous studies revealed that ovary formation in A. altiparanae occurred at 58 dph, whereas the first sign of testis formation was found at 73 dph. In relation to the control, E2 exposure increased the proportion of phenotypic females in 120% and 148.4% for 4 and 6 mg E2/Kg, respectively. However, histological analysis revealed that treatments did not affect gonadal sex ratio between males and females, but induced intersex (testisova) in the group treated with 6 mg E2/Kg food. Treatment with E2 also altered gonadal transcript levels of a selected number of genes implicated in sexual differentiation. Males overexpressed dmrt1, sox9 and amh following E2 treatment as compared to control. Females showed increased mRNA levels of dmrtl and sox9, which might be related to the down-regulation of cyp19a1a after E2 exposure. In summary, E2 exposure during early gonadal development affected male secondary characteristics without changing the gonadal sex ratio, and altered expression of genes implicated in sexual differentiation. (AU)

Processo FAPESP: 14/07620-7 - Banco de espermatogônias tronco em peixes para conservação do genótipo de espécies ameaçadas e de interesse econômico
Beneficiário:Rafael Henrique Nóbrega
Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores
Processo FAPESP: 16/12101-4 - Seleção, Cultura e Transcriptoma de espermatogônias tronco de zebrafish, Danio rerio: uma abordagem funcional e genômica
Beneficiário:Lucas Benites Doretto
Linha de fomento: Bolsas no Brasil - Mestrado
Processo FAPESP: 18/10265-5 - Mecanismos endócrinos e moleculares envolvidos na diferenciação sexual de medaka (Oryzias latipes) em condições de estresse térmico
Beneficiário:Ivana Felipe da Rosa
Linha de fomento: Bolsas no Brasil - Mestrado
Processo FAPESP: 14/25313-4 - O papel do AMH na diferenciação sexual e na gametogênese de peixes teleósteos
Beneficiário:Marcos Antônio de Oliveira
Linha de fomento: Bolsas no Brasil - Doutorado