Canonical and Non-canonical Inflammasome Activation by Outer Membrane Vesicles Derived FromBordetella pertussis

Autor(es)

Elizagaray, Maia L. ^[1] ; Gomes, Marco Tulio R. ^[2] ; Guimaraes, Erika S. ^{[2, 3]} ; Rumbo, Martin ^[1] ; Hozbor, Daniela F. ^[4] ; Oliveira, Sergio C. ^[2] ; Moreno, Griselda ^[1]

Afiliação do(s) autor(es)

^[1] UNLP, CONICET, Fac Ciencias Exactas, Inst Estudios Inmunol & Fisiopatol IIFP, La Plata - Argentina

^[2] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Bioquim & Imunol, Belo Horizonte, MG - Brazil

^[3] Univ Fed Minas Gerais, Inst Ciencias Biol, Programa Posgrad Genet, Belo Horizonte, MG - Brazil

^[4] Univ Nacl La Plata, CCT CONICET La Plata, Inst Biotecnol & Biol Mol IBBM, Fac Ciencias Exactas, Lab VacSal, La Plata - Argentina

• Tipo de documento: Artigo Científico
• Fonte: FRONTIERS IN IMMUNOLOGY; v. 11, AUG 20 2020.
• Citações Web of Science: 0

Resumo

Outer Membrane Vesicles (OMVs) derived from different Gram-negative bacteria have been proposed as an attractive vaccine platform because of their own immunogenic adjuvant properties. Pertussis or whooping cough is a highly contagious vaccine-preventable respiratory disease that resurged during the last decades in many countries. In response to the epidemiological situation, new boosters have been incorporated into vaccination schedules worldwide and new vaccine candidates have started to be designed. Particularly, our group designed a new pertussis vaccine candidate based on OMVs derived fromBordetella pertussis(BpOMVs). To continue with the characterization of the immune response induced by our OMV based vaccine candidate, this work aimed to investigate the ability of OMVs to activate the inflammasome pathway in macrophages. We observed that NLRP3, caspase-1/11, and gasdermin-D (GSDMD) are involved in inflammasome activation by BpOMVs. Moreover, we demonstrated that BpOMVs as well as transfectedB. pertussislipooligosaccharide (BpLOS) induce caspase-11 (Casp11) and guanylate-binding proteins (GBPs) dependent non-canonical inflammasome activation. Our results elucidate the mechanism by which BpOMVs trigger one central pathway of the innate response activation that is expected to skew the adaptive immune response elicited by BpOMVs vaccination. (AU)

• Processo FAPESP: 17/24832-6 - Desenvolvimento de vacinas baseadas em BCG recombinante: Tuberculose, Pertussis, Pneumococo e Schistosoma
• Beneficiário: Luciana Cezar de Cerqueira Leite
• Modalidade de apoio: Auxílio à Pesquisa - Temático