Composites of Crosslinked Aggregates of Eversa(R)Transform and Magnetic Nanoparticles. Performance in the Ethanolysis of Soybean Oil

Autor(es)

Miranda, Leticia Passos ^[1] ; Guimaraes, Jose Renato ^[1] ; Giordano, Roberto Campos ^[1] ; Fernandez-Lafuente, Roberto ^[2] ; Tardioli, Paulo Waldir ^[1]

Afiliação do(s) autor(es)

^[1] Fed Univ Sao Carlos DEQ UFSCar, Dept Chem Engn, Postgrad Program Chem Engn PPGEQ, Rod Washington Luis, Km 235, BR-13565905 Sao Carlos, SP - Brazil

^[2] CSIC, ICP, Dept Biocatalisis, Campus UAM CSIC, Madrid 28049 - Spain

• Tipo de documento: Artigo Científico
• Fonte: CATALYSTS; v. 10, n. 8 AUG 2020.
• Citações Web of Science: 0

Resumo

Eversa(R)Transform 2.0 has been launched to be used in free form, but its immobilization may improve its performance. This work aimed to optimize the immobilization of Eversa(R)Transform 2.0 by the crosslinked enzyme aggregates (CLEAs) technique, using almost all the available tools to improve its performance. Several variables in the CLEA preparation were optimized to improve the recovered activity, such as precipitant nature and crosslinker concentration. Moreover, some feeders were co-precipitated to improve the crosslinking step, such as bovine serum albumin, soy protein, or polyethyleneimine. Starch (later enzymatically degraded) was utilized as a porogenic agent to decrease the substrate diffusion limitations. Silica magnetic nanoparticles were also utilized to simplify the CLEA handling, but it was found that a large percentage of the Eversa activity could be immobilized on these nanoparticles before aggregation. The best CLEA protocol gave a 98.9% immobilization yield and 30.1% recovered activity, exhibited a porous structure, and an excellent performance in the transesterification of soybean oil with ethanol: 89.8 wt% of fatty acid ethyl esters (FAEEs) yield after 12 h of reaction, while the free enzyme required a 48 h reaction to give the same yield. A caustic polishing step of the product yielded a biodiesel containing 98.9 wt% of FAEEs and a free fatty acids content lower than 0.25%, thus the final product met the international standards for biodiesel. The immobilized biocatalyst could be reused for at least five 12 h-batches maintaining 89.6% of the first-batch yield, showing the efficient catalyst recovery by applying an external magnetic field. (AU)

• Processo FAPESP: 16/10636-8 - Da fábrica celular à biorrefinaria integrada Biodiesel-Bioetanol: uma abordagem sistêmica aplicada a problemas complexos em micro e macroescalas
• Beneficiário: Roberto de Campos Giordano
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Temático