Transdermal permeation of bacteriophage particles by choline oleate: potential for treatment of soft-tissue infections

Autor(es)

Campos, Welida F. ^[1] ; Silva, Erica C. ^[1] ; Oliveira, Thais J. ^[1] ; Oliveira, Jr., Jose M. ^[1] ; Tubino, Matthieu ^[2] ; Pereira, Carla ^{[3, 4]} ; Vila, Marta M. D. C. ^[1] ; Balcao, Victor M. ^{[3, 4, 1]}

Afiliação do(s) autor(es)

^[1] Univ Sorocaba, PhageLab Lab Biofilms & Bacteriophages, BR-18023000 Sorocaba, SP - Brazil

^[2] Univ Estadual Campinas, Inst Chem, Campinas, SP - Brazil

^[3] Univ Aveiro, Dept Biol, Campus Univ Santiago, P-3810193 Aveiro - Portugal

^[4] Univ Aveiro, CESAM, Campus Univ Santiago, P-3810193 Aveiro - Portugal

• Tipo de documento: Artigo Científico
• Fonte: FUTURE MICROBIOLOGY; v. 15, n. 10, p. 881-896, AUG 2020.
• Citações Web of Science: 0

Resumo

Aim: A sodium alginate-based biohydrogel was prepared integrating choline oleate deep euthetic solvent as facilitator of transdermal delivery and a cocktail of lytic bacteriophages for Acinetobacter baumannii, aiming at treating soft-tissue infections by the aforementioned pathogen. Materials \& methods: Two bacteriophages were isolated from a hospital sewage and a wastewater treatment plant sewage in Sorocaba (Brazil), and characterized via SDS-PAGE electrophoresis, transmission electron microscope and evaluation of lytic spectra of the bacteriophage cocktail. The biohydrogel was prepared and characterized by DSC, FTIR, XRD, DESEM, XRT and transdermal permeation of the bacteriophage cocktail. Results \& conclusion: The physico-chemical characterization of the biohydrogel produce indicated adequate structural characteristics and ability to promote/facilitate transdermal delivery of bacteriophage particles, thus showing potential for biopharmaceutical applications. (AU)

• Processo FAPESP: 16/08884-3 - PneumoPhageColor - desenvolvimento de um kit de biodetecção colorimétrica de Pseudomonas aeruginosa com base em partículas fágicas
• Beneficiário: Vitor Manuel Cardoso Figueiredo Balcão
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 18/05522-9 - PsaPhageKill - isolamento, caracterização e uso de bacteriófagos líticos contra Pseudomonas syringae pv. actinidiae para combater o cancro do kiwi: uma alternativa eficiente e amiga do ambiente
• Beneficiário: Vitor Manuel Cardoso Figueiredo Balcão
• Modalidade de apoio: Bolsas no Exterior - Pesquisa
• Processo FAPESP: 16/12234-4 - TransAppIL - Permeação cutânea de entidades proteicas estabilizadas estruturalmente e funcionalmente, carreadas por líquidos iônicos.
• Beneficiário: Marta Maria Duarte Carvalho Vila
• Modalidade de apoio: Auxílio à Pesquisa - Regular