Transdermal permeation of bacteriophage particles by choline oleate: potential for treatment of soft-tissue infections

Author(s)

Campos, Welida F. ^[1] ; Silva, Erica C. ^[1] ; Oliveira, Thais J. ^[1] ; Oliveira, Jr., Jose M. ^[1] ; Tubino, Matthieu ^[2] ; Pereira, Carla ^{[3, 4]} ; Vila, Marta M. D. C. ^[1] ; Balcao, Victor M. ^{[3, 4, 1]}

Affiliation

^[1] Univ Sorocaba, PhageLab Lab Biofilms & Bacteriophages, BR-18023000 Sorocaba, SP - Brazil

^[2] Univ Estadual Campinas, Inst Chem, Campinas, SP - Brazil

^[3] Univ Aveiro, Dept Biol, Campus Univ Santiago, P-3810193 Aveiro - Portugal

^[4] Univ Aveiro, CESAM, Campus Univ Santiago, P-3810193 Aveiro - Portugal

• Document type: Journal article
• Source: FUTURE MICROBIOLOGY; v. 15, n. 10, p. 881-896, AUG 2020.
• Web of Science Citations: 0

Abstract

Aim: A sodium alginate-based biohydrogel was prepared integrating choline oleate deep euthetic solvent as facilitator of transdermal delivery and a cocktail of lytic bacteriophages for Acinetobacter baumannii, aiming at treating soft-tissue infections by the aforementioned pathogen. Materials \& methods: Two bacteriophages were isolated from a hospital sewage and a wastewater treatment plant sewage in Sorocaba (Brazil), and characterized via SDS-PAGE electrophoresis, transmission electron microscope and evaluation of lytic spectra of the bacteriophage cocktail. The biohydrogel was prepared and characterized by DSC, FTIR, XRD, DESEM, XRT and transdermal permeation of the bacteriophage cocktail. Results \& conclusion: The physico-chemical characterization of the biohydrogel produce indicated adequate structural characteristics and ability to promote/facilitate transdermal delivery of bacteriophage particles, thus showing potential for biopharmaceutical applications. (AU)

• FAPESP's process: 16/08884-3 - PneumoPhageColor - development of a colorimetric biodetection kit for Pseudomonas aeruginosa based on phage particles
• Grantee: Vitor Manuel Cardoso Figueiredo Balcão
• Support Opportunities: Regular Research Grants
• FAPESP's process: 18/05522-9 - PsaPhageKill - isolation, characterisation and use of lytic bacteriophages against Pseudomonas syringae PV. actinidiae to fight the cancer of kiwifruit: an efficient and ecofriendly alternative
• Grantee: Vitor Manuel Cardoso Figueiredo Balcão
• Support Opportunities: Scholarships abroad - Research
• FAPESP's process: 16/12234-4 - TransAppIL - Transdermal delivery of structurally and functionally stabilized protein entities applying ionic liquids.
• Grantee: Marta Maria Duarte Carvalho Vila
• Support Opportunities: Regular Research Grants