Resveratrol and insulin association reduced alveolar bone loss and produced an antioxidant effect in diabetics rats

Autor(es)

Cirano, Fabiano Ribeiro ^[1] ; Molez, Andreia Manetta ^[1] ; Ribeiro, Fernanda Vieira ^[1] ; Tenenbaum, Howard C. ^{[2, 3, 4, 5, 6]} ; Casati, Marcio Z. ^[1] ; Correa, Monica Grazieli ^[1] ; Pimentel, Suzana Peres ^[1]

Afiliação do(s) autor(es)

^[1] Univ Paulista, Sch Dent, Dent Res Div, Sao Paulo - Brazil

^[2] Fac Dent, Dept Periodontol, Toronto, ON - Canada

^[3] Univ Toronto, Lab Med & Pathobiol, Fac Med, Toronto, ON - Canada

^[4] Tel Aviv Univ, Sch Dent Med, Dept Periodont, Tel Aviv - Israel

^[5] Sinai Hlth Syst, Dept Dent, Toronto, ON - Canada

^[6] Sinai Hlth Syst, Ctr Adv Dent Res & Care, Toronto, ON - Canada

• Tipo de documento: Artigo Científico
• Fonte: Journal of Periodontology; v. 92, n. 5 SEP 2020.
• Citações Web of Science: 0

Resumo

Background The present investigation studied the effects of systemic administration of resveratrol (RSV) on the development of experimental periodontitis (EP) and on the release of markers of inflammation, bone metabolism, and oxidative stress in diabetic rats. Methods Seventy-five male rats were divided into five groups: DM+PLAC: Diabetes Mellitus + placebo solution; DM+INS: DM + insulin therapy; DM+RSV: DM + RSV; DM+RSV+INS: DM + RSV and insulin; NDM: non-diabetic. Streptozotocin was used to induce DM and EP was induced by the placement of a ligature at the fist mandibular and the second maxillary molars. Euthanasia occurred 30 days after the initiation of the study and mandible specimens were subjected for morphometric analysis of bone level. Gingival tissues from mandibular molars were collected for quantification of inflammatory and oxidative stress markers by multiplex assay system and ELISA assay, respectively. Maxillary gingival tissues were processed for real-time polymerase chain reaction (real-time PCR) assessment of markers of bone metabolism and oxidative stress. Results Morphometric analysis revealed greater bone loss in DM+PLAC and DM+INS in comparison to the other treatments (P < 0.05). RSV used in conjunction with INS reduced the levels of interleukin (IL)-1 beta, IL-6, IL-17, interferon-gamma (IFN-gamma) and superoxide dismutase 1 (SOD) (P < 0.05). RSV alone reduced nicotinamide adenine dinucleotide phosphatase oxidase (NADPH oxidase) levels, in comparison to DM+INS and DM+RSV+INS (P < 0.05). All treatments upregulated mRNA levels for osteoprotegerin (OPG) in comparison to PLAC (P < 0.05). Sirtuin 1 (SIRT) mRNA levels were lower in PLAC when compared to DM+RSV, DM+RSV+INS and NDM (P < 0.05). Conclusion RSV reduced the progression of EP and the levels of NADPH oxidase. Co-treatment with RSV and insulin reduced the levels of pro-inflammatory factors (either proteins or mRNA) and increased the levels of SOD. The data also demonstrated that treatment with RSV and INS alone or in combination had beneficial effects on bone loss. (AU)

• Processo FAPESP: 15/13894-5 - Reserva Técnica para Infraestrutura Institucional de Pesquisa
• Beneficiário: Fernanda Vieira Ribeiro
• Modalidade de apoio: Auxílio à Pesquisa - Reserva Técnica para Infraestrutura Institucional de Pesquisa