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(Referência obtida automaticamente do SciELO, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Hybrid Hydrogels Based on Polyethylene Glycol Bioconjugated with Silylated-Amyloidogenic Peptides

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Autor(es):
Carla C. Decandio [1] ; Sandra V. Vassiliades [2] ; Barbara B. Gerbelli [3] ; Andrea M. Aguilar [4] ; Wendel A. Alves [5]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Universidade Federal do ABC. Centro de Ciências Naturais e Humanas - Brasil
[2] Universidade Federal do ABC. Centro de Ciências Naturais e Humanas - Brasil
[3] Universidade Federal do ABC. Centro de Ciências Naturais e Humanas - Brasil
[4] Universidade Federal de São Paulo. Instituto de Ciências Ambientais, Químicas e Farmacêuticas - Brasil
[5] Universidade Federal do ABC. Centro de Ciências Naturais e Humanas - Brasil
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: Journal of the Brazilian Chemical Society; v. 31, n. 11, p. 2200-2210, 2020-10-30.
Resumo

Hybrid-peptide hydrogel arrangements are a promising alternative to obtaining biocompatible structures at the nanoscopic scale. In this work, a new class of hybrid hydrogels was obtained through the sol-gel process based on the reaction between an amyloid-like octapeptide sequence [RF]4 (where R = arginine and F = phenylalanine) covalently bonded with glycine-linked alkoxysilanes, and hybrid silylated polyethylene glycol (PEG). 1H nuclear magnetic resonance (NMR), liquid chromatography-mass spectrometry (LC-MS), and Fourier transform infrared spectroscopy (FTIR) analyses were used, resulting in synthesized precursor/intermediate molecules. The structure of hybrid hydrogel fibers was studied by atomic force microscopy (AFM) and small-angle X-ray scattering (SAXS), where the existence of two regions in the Guinier plot was observed, one being predominantly formed by polymer while the other one by peptide chains. The rheological measurements showed that viscoelastic parameters depend on both the amount of silane peptide in the hydrogel matrix and increased temperature. Also, the FTIR spectra indicated the coexistence of antiparallel and parallel β-sheet structure patterns into amyloid fibril hydrogels, which can be modulated by peptide-silane coupling in each formulation. The analyzed hydrogels showed thixotropic and shear-thinning rheology at physiological pH, leaving open the opportunity to topical drug delivery system applications in the future. (AU)

Processo FAPESP: 14/50867-3 - INCT 2014: Instituto Nacional de Ciência e Tecnologia de Bioanalítica
Beneficiário:Marco Aurelio Zezzi Arruda
Modalidade de apoio: Auxílio à Pesquisa - Temático
Processo FAPESP: 17/02317-2 - Interfaces em materiais: propriedades eletrônicas, magnéticas, estruturais e de transporte
Beneficiário:Adalberto Fazzio
Modalidade de apoio: Auxílio à Pesquisa - Temático