Hybrid Hydrogels Based on Polyethylene Glycol Bioconjugated with Silylated-Amyloidogenic Peptides

Author(s)

Carla C. Decandio ^[1] ; Sandra V. Vassiliades ^[2] ; Barbara B. Gerbelli ^[3] ; Andrea M. Aguilar ^[4] ; Wendel A. Alves ^[5]

Affiliation

^[1] Universidade Federal do ABC. Centro de Ciências Naturais e Humanas - Brasil

^[2] Universidade Federal do ABC. Centro de Ciências Naturais e Humanas - Brasil

^[3] Universidade Federal do ABC. Centro de Ciências Naturais e Humanas - Brasil

^[4] Universidade Federal de São Paulo. Instituto de Ciências Ambientais, Químicas e Farmacêuticas - Brasil

^[5] Universidade Federal do ABC. Centro de Ciências Naturais e Humanas - Brasil

• Document type: Journal article
• Source: Journal of the Brazilian Chemical Society; v. 31, n. 11, p. 2200-2210, 2020-10-30.

Abstract

Hybrid-peptide hydrogel arrangements are a promising alternative to obtaining biocompatible structures at the nanoscopic scale. In this work, a new class of hybrid hydrogels was obtained through the sol-gel process based on the reaction between an amyloid-like octapeptide sequence [RF]4 (where R = arginine and F = phenylalanine) covalently bonded with glycine-linked alkoxysilanes, and hybrid silylated polyethylene glycol (PEG). 1H nuclear magnetic resonance (NMR), liquid chromatography-mass spectrometry (LC-MS), and Fourier transform infrared spectroscopy (FTIR) analyses were used, resulting in synthesized precursor/intermediate molecules. The structure of hybrid hydrogel fibers was studied by atomic force microscopy (AFM) and small-angle X-ray scattering (SAXS), where the existence of two regions in the Guinier plot was observed, one being predominantly formed by polymer while the other one by peptide chains. The rheological measurements showed that viscoelastic parameters depend on both the amount of silane peptide in the hydrogel matrix and increased temperature. Also, the FTIR spectra indicated the coexistence of antiparallel and parallel β-sheet structure patterns into amyloid fibril hydrogels, which can be modulated by peptide-silane coupling in each formulation. The analyzed hydrogels showed thixotropic and shear-thinning rheology at physiological pH, leaving open the opportunity to topical drug delivery system applications in the future. (AU)

• FAPESP's process: 17/02317-2 - Interfaces in materials: electronic, magnetic, structural and transport properties
• Grantee: Adalberto Fazzio
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 14/50867-3 - INCT 2014: National Institute of Science and Technology in Bioanalysis
• Grantee: Marco Aurelio Zezzi Arruda
• Support Opportunities: Research Projects - Thematic Grants