Statherin-derived peptide protects against intrinsic erosion

Autor(es)

Taira, Even Akemi ^[1] ; Carvalho, Gabriel ^[1] ; Ferrari, Carolina Ruis ^[1] ; Martini, Tatiana ^[1] ; Pela, Vinicius Taioqui ^[1] ; Oliveira Ventura, Talita Mendes ^[1] ; Dionizio, Aline Salgado ^[1] ; Crusca, Edson ^[2] ; Marchetto, Reinaldo ^[2] ; Rabelo Buzalaf, Marilia Afonso ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Bauru Sch Dent, Bauru, SP - Brazil

^[2] Univ Estadual Paulista, Chem Inst, Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ARCHIVES OF ORAL BIOLOGY; v. 119, NOV 2020.
• Citações Web of Science: 0

Resumo

Objectives: In the present study, we used an in vitro initial intrinsic erosion model to evaluate: (experiment 1) the influence of the degree of serine (Ser) phosphorylation of peptides containing the 15 N-terminal residues of statherin and (experiment 2) the effect of different concentrations of the peptide with the best performance in experiment 1 on initial enamel erosion. Design: Bovine enamel specimens were divided into 6 groups (n = 15/group) for each experiment. In experiment 1, the peptides evaluated (at 1.88 x 10(-5) M) were: not phosphorylated (StatSS), phosphorylated in Ser2 (StatpSS), phosphorylated in Ser3 (StatSpS) phosphorylated in Ser2 and Ser3 (StatpSpS). Phosphate buffer and human recombinant statherin were used as negative and positive controls, respectively. In experiment 2, StatpSpS was evaluated at different concentrations: 0.94, 1.88, 3.76 and 7.52 x 10(-5) M. Phosphate buffer and 0.1 mg/mL CaneCPI-5 were employed as negative and positive controls, respectively. In each experiment, the specimens were incubated with the solutions for 2 h, then the AEP was allowed to form (under human pooled saliva) for 2 h. The specimens were then challenged with 0.01 M HCl for 10 s. Demineralization was evaluated by percentage of surface hardness change (%SHC). Data were analyzed by ANOVA and Tukey's test (p < 0.05). Results: In experiment 1, only StatpSpS significantly reduced the % SHC in comparison with control. In experiment 2, 1.88 x 10(-5) M StatpSpS significantly reduced the %SHC in comparison with control. Conclusions: This is the first study showing that statherin-derived peptide might protect against intrinsic erosion. (AU)

• Processo FAPESP: 18/18749-1 - Avaliação in vitro do potencial protetor contra a erosão dentária inicial da modificação da película adquirida do esmalte com soluções contendo estaterina ou peptídeos dela derivados, fosforilados em serina ou não
• Beneficiário: Gabriel Carvalho
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica