Comparison of cell invasion, macrophage survival and inflammatory cytokines profiles between Salmonella enterica serovars Enteritidis and Dublin from Brazil

Autor(es)

Campioni, F. ^[1] ; Gomes, C. N. ^[1] ; Bergamini, A. M. M. ^[2] ; Rodrigues, D. P. ^[3] ; Tiba-Casas, M. R. ^[4] ; Falcao, J. P. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Anal Clin Toxicol & Bromatol, Ribeirao Preto, SP - Brazil

^[2] Adolfo Lutz Inst, Ctr Lab Reg Ribeirao Preto VI, Lab Microbiol Alimentos, Ribeirao Preto, SP - Brazil

^[3] Fiocruz MS, Fdn Oswaldo Cruz, Lab Enterobacterias, Rio De Janeiro, RJ - Brazil

^[4] Adolfo Lutz Inst, Ctr Bacteriol, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Applied Microbiology; v. 130, n. 6 NOV 2020.
• Citações Web of Science: 1

Resumo

Aims This study compared the capacity of strains of Salmonella enterica serovars Enteritidis and Dublin isolated in Brazil to invade epithelial cells, to be internalized by and survive within macrophages, and to stimulate cytokine release in vitro. Methods and Results Both serovars infected 75 and 73% Caco-2 (human) and MDBK (bovine) epithelial cells respectively. Salmonella Dublin and S. Enteritidis (i) were internalized at the respective rates of 79 center dot 6 and 65 center dot 0% (P <= 0 center dot 05) by U937 (human) macrophages, and 70 center dot 4 and 66 center dot 9% by HD11 (chicken) macrophages; and (ii) multiplied at the respective rates of 3 center dot 2- and 2 center dot 7-fold within U937 cells, and 1 center dot 9- and 1 center dot 1-fold (P <= 0 center dot 05) within HD11 cells respectively. Seventy per cent of 10 S. Dublin strains stimulated IL-8 production, while 70% of S. Enteritidis strains enhanced production of IL-1 beta, IL-6, IL-8, IL-10, IL-12p70 and TNF in Caco-2 cells. Conclusions Compared with S. Enteritidis, S. Dublin had stronger ability to survive within macrophages and induced weak cytokine production, which may explain the higher incidence of invasive diseases caused by S. Dublin in humans. Significance and Impact of the STUDY This study compared S. enterica serovars Enteritidis and Dublin to provide comparative data about the profile of the two serovars in cells from humans, the common host and their respective natural animal hosts and vice versa in order to check the differences between these two phylogenetically closely related serovars that share antigenic properties but present different phenotypic behaviours. (AU)

• Processo FAPESP: 16/24716-3 - Sequenciamento do genoma, transcriptoma e análise fenotípica de linhagens de Campylobacter coli isoladas de diversas fontes no Brasil
• Beneficiário: Juliana Pfrimer Falcão
• Modalidade de apoio: Auxílio à Pesquisa - Regular