Impact of Atrazine Exposure on the Microbial Community Structure in a Brazilian Tropical Latosol Soil

Autor(es)

Tonelli Fernandes, Ana Flavia ^{[1, 2]} ; Wang, Ping ^[2] ; Staley, Christopher ^[2] ; Silva Moretto, Jessica Aparecida ^[1] ; Altarugio, Lucas Miguel ^[3] ; Campanharo, Sarah Chagas ^[1] ; Stehling, Eliana Guedes ^[1] ; Sadowsky, Michael Jay ^{[2, 4, 5]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Pharmaceut Sci Ribeirao Preto, Cafe Ave S-N, BR-14040903 Ribeirao Preto, SP - Brazil

^[2] Univ Minnesota, Biotechnol Inst, 1479 Gortner Ave, St Paul, MN 55108 - USA

^[3] Univ Sao Paulo, Dept Soil Sci ESALQ, 11 Padua Dias Ave, BR-13418260 Piracicaba, SP - Brazil

^[4] Univ Minnesota, Dept Soil Water & Climate, 1991 Upper Buford Circle, St Paul, MN 55108 - USA

^[5] Univ Minnesota, Dept Plant & Microbial Biol, 1479 Gortner Avenue, St Paul, MN 55108 - USA

• Tipo de documento: Artigo Científico
• Fonte: MICROBES AND ENVIRONMENTS; v. 35, n. 2 2020.
• Citações Web of Science: 3

Resumo

Atrazine is a triazine herbicide that is widely used to control broadleaf weeds. Its widespread use over the last 50 years has led to the potential contamination of soils, groundwater, rivers, and lakes. Its main route of complete degradation is via biological means, which is carried out by soil microbiota using a 6-step pathway. The aim of the present study was to investigate whether application of atrazine to soil changes the soil bacterial community. We used 16S rRNA gene sequencing and qPCR to elucidate the microbial community structure and assess the abundance of the atrazine degradation genes atzA, atzD, and trzN in a Brazilian soil. The results obtained showed that the relative abundance of atzA and trzN, encoding triazinc-initiating metabolism in Gram-negative and -positive bacteria, respectively, increased in soil during the first weeks following the application of atrazine. In contrast, the abundance of atzD, encoding cyanuric acid amidohydrolase -the fourth step in the pathway-was not related to the atrazine treatment. Moreover, the overall soil bacterial community showed no significant changes after the application of atrazine. Despite this, we observed increases in the relative abundance of bacterial families in the 4th and 8th weeks following the atrazine treatment, which may have been related to higher copy numbers of atzA and trzN, in part due to the release of nitrogen from the herbicide. The present results revealed that while the application of atrazine may temporarily increase the quantities of the atz4 and trzN genes in a Brazilian Red Latosol soil, it does not lead to significant and long-term changes in the bacterial community structure. (AU)

• Processo FAPESP: 15/18990-2 - Investigação dos mecanismos de resistência às quinolonas em isolados bacterianos ambientais
• Beneficiário: Eliana Guedes Stehling
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/03982-0 - Avaliação da comunidade microbiana de solos contaminados por atrazina
• Beneficiário: Ana Flavia Tonelli Scaranello
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado