MALDI-TOF MS and genomic analysis can make the difference in the clarification of canine brucellosis outbreaks

Autor(es)

da Silva, David Attuy Vey ^{[1, 2]} ; Brendebach, Holger ^[2] ; Gruetzke, Josephine ^[2] ; Dieckmann, Ralf ^[2] ; Soares, Rodrigo Martins ^[1] ; Ribeiro de Lima, Julia Teresa ^[1] ; Keid, Lara Borges ^[3] ; Hofreuter, Dirk ^[2] ; Al Dahouk, Sascha ^{[2, 4]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Vet Med & Anim Sci, Dept Prevent Vet Med & Anim Hlth, Sao Paulo - Brazil

^[2] German Fed Inst Risk Assessment, Dept Biol Safety, Berlin - Germany

^[3] Univ Sao Paulo, Fac Anim Sci & Food Engn, Dept Vet Med, Pirassununga - Brazil

^[4] RWTH Aachen Univ Hosp, Dept Internal Med, Aachen - Germany

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 10, n. 1 NOV 6 2020.
• Citações Web of Science: 2

Resumo

Brucellosis is one of the most common bacterial zoonoses worldwide affecting not only livestock and wildlife but also pets. Canine brucellosis is characterized by reproductive failure in dogs. Human Brucella canis infections are rarely reported but probably underestimated due to insufficient diagnostic surveillance. To improve diagnostics, we investigated dogs in a breeding kennel that showed clinical manifestations of brucellosis and revealed positive blood cultures. As an alternative to the time-consuming and hazardous classical identification procedures, a newly developed species-specific intact-cell matrix-assisted laser desorption/ionization-time of flight mass spectrometry analysis was applied, which allowed for rapid identification of B. canis and differentiation from closely related B. suis biovar 1. High-throughput sequencing and comparative genomics using single nucleotide polymorphism analysis clustered our isolates together with canine and human strains from various Central and South American countries in a distinct sub-lineage. Hence, molecular epidemiology clearly defined the outbreak cluster and demonstrated the endemic situation in South America. Our study illustrates that MALDI-TOF MS analysis using a validated in-house reference database facilitates rapid B. canis identification at species level. Additional whole genome sequencing provides more detailed outbreak information and leads to a deeper understanding of the epidemiology of canine brucellosis. (AU)

• Processo FAPESP: 16/01276-8 - Caracterização imunoproteômica de Brucella canis para identificação de proteínas candidatas a antígenos para sorodiagnóstico
• Beneficiário: David Attuy Vey da Silva
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 16/13804-9 - Caracterização imunoproteômica de Brucella canis para identificação de proteínas candidatas a antígenos para sorodiagnóstico
• Beneficiário: David Attuy Vey da Silva
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado