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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Oxidative origin of sperm DNA fragmentation in the adult varicocele

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Autor(es):
Jeremias, Jessica Timoteo [1] ; Belardin, Larissa Berloffa [1] ; Okada, Fatima Kazue [1] ; Antoniassi, Mariana P. [1] ; Fraietta, Renato [1] ; Bertolla, Ricardo Pimenta [1] ; Intasqui, Paula [1]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Univ Fed Sao Paulo Unifesp, Dept Cirurgia, Div Urol, Secao Reprod Humana, Sao Paulo, SP - Brazil
Número total de Afiliações: 1
Tipo de documento: Artigo Científico
Fonte: INTERNATIONAL BRAZ J UROL; v. 47, n. 2, p. 275-283, MAR-APR 2021.
Citações Web of Science: 1
Resumo

ABSTRACT Purpose: Sperm DNA fragmentation is a major cellular mechanism underlying varicocele-related male infertility. However, the type of DNA fragmentation - whether oxidative or of another nature - remains unknown. Thus, the aim of this study was to evaluate single- and double-stranded sperm DNA fragmentation, and oxidative-induced sperm DNA damage in men with varicocele. Materials and Methods: A cross-sectional study was performed, including 94 normozoospermic adults, of which 39 men without varicocele (controls) and 55 men with varicocele grades II or III, uni- or bilaterally. All men collected semen by masturbation. After semen analysis, the remaining volume was used for evaluation of three types of sperm DNA damage: (i) total DNA fragmentation, using an alkaline comet assay, (ii) double-stranded DNA fragmentation, using a neutral comet assay, and (iii) oxidative DNA damage, using an alkaline comet assay associated with the DNA glycosylase formamidopyrimidine enzyme. In each assay, percentage of sperm with any degree of DNA fragmentation, and with high DNA fragmentation were compared between the groups using an unpaired Student's t test or a Mann-Whitney test. Results: The varicocele group presented a higher rate of sperm with fragmented DNA (both any and high DNA fragmentation), considering single-stranded DNA fragmentation, double-stranded DNA fragmentation, or a combination of both, as well as oxidative- induced DNA fragmentation. Conclusions: Patients with varicocele have an increase in sperm DNA fragmentation levels, particularly in oxidative stress-induced sperm DNA damage. (AU)

Processo FAPESP: 19/05879-7 - Efeito da exposição ao material particulado inalável fino (MP2,5) na qualidade funcional de espermatozoides de camundongos
Beneficiário:Paula Intasqui Lopes
Modalidade de apoio: Auxílio à Pesquisa - Regular
Processo FAPESP: 14/17185-6 - Análise proteômica confirmatória de biomarcadores de alterações funcionais dos espermatozóides e estresse oxidativo seminal
Beneficiário:Ricardo Pimenta Bertolla
Modalidade de apoio: Auxílio à Pesquisa - Regular
Processo FAPESP: 14/11493-0 - Análise proteômica confirmatória de um painel de biomarcadores seminais de alterações funcionais dos espermatozoides e de estresse oxidativo seminal
Beneficiário:Paula Intasqui Lopes
Modalidade de apoio: Bolsas no Brasil - Doutorado