| Full text | |
| Author(s): |
Jeremias, Jessica Timoteo
[1]
;
Belardin, Larissa Berloffa
[1]
;
Okada, Fatima Kazue
[1]
;
Antoniassi, Mariana P.
[1]
;
Fraietta, Renato
[1]
;
Bertolla, Ricardo Pimenta
[1]
;
Intasqui, Paula
[1]
Total Authors: 7
|
| Affiliation: | [1] Univ Fed Sao Paulo Unifesp, Dept Cirurgia, Div Urol, Secao Reprod Humana, Sao Paulo, SP - Brazil
Total Affiliations: 1
|
| Document type: | Journal article |
| Source: | INTERNATIONAL BRAZ J UROL; v. 47, n. 2, p. 275-283, MAR-APR 2021. |
| Web of Science Citations: | 1 |
| Abstract | |
ABSTRACT Purpose: Sperm DNA fragmentation is a major cellular mechanism underlying varicocele-related male infertility. However, the type of DNA fragmentation - whether oxidative or of another nature - remains unknown. Thus, the aim of this study was to evaluate single- and double-stranded sperm DNA fragmentation, and oxidative-induced sperm DNA damage in men with varicocele. Materials and Methods: A cross-sectional study was performed, including 94 normozoospermic adults, of which 39 men without varicocele (controls) and 55 men with varicocele grades II or III, uni- or bilaterally. All men collected semen by masturbation. After semen analysis, the remaining volume was used for evaluation of three types of sperm DNA damage: (i) total DNA fragmentation, using an alkaline comet assay, (ii) double-stranded DNA fragmentation, using a neutral comet assay, and (iii) oxidative DNA damage, using an alkaline comet assay associated with the DNA glycosylase formamidopyrimidine enzyme. In each assay, percentage of sperm with any degree of DNA fragmentation, and with high DNA fragmentation were compared between the groups using an unpaired Student's t test or a Mann-Whitney test. Results: The varicocele group presented a higher rate of sperm with fragmented DNA (both any and high DNA fragmentation), considering single-stranded DNA fragmentation, double-stranded DNA fragmentation, or a combination of both, as well as oxidative- induced DNA fragmentation. Conclusions: Patients with varicocele have an increase in sperm DNA fragmentation levels, particularly in oxidative stress-induced sperm DNA damage. (AU) | |
| FAPESP's process: | 19/05879-7 - Effect of the exposure to fine particulate matter (PM2.5) on sperm functional quality of mice |
| Grantee: | Paula Intasqui Lopes |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 14/17185-6 - Confirmatory proteomic analysis of biomarkers of sperm functional alteration and seminal oxidative stress |
| Grantee: | Ricardo Pimenta Bertolla |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 14/11493-0 - Confirmatory proteomic analysis of a panel of seminal biomarkers of sperm functional abnormalities and semen oxidative stress |
| Grantee: | Paula Intasqui Lopes |
| Support Opportunities: | Scholarships in Brazil - Doctorate |