| Texto completo | |
| Autor(es): |
Mallaupoma Camarena, Denisse Esther
[1]
;
Alcantara Sekimoto Matsuyama, Larissa Satiko
[2]
;
Maria-Engler, Silvya Stuchi
[2]
;
Catalani, Luiz Henrique
[1]
Número total de Autores: 4
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Dept Fundamental Chem, Inst Chem, Lab Polymer Biomat, BR-05508000 Sao Paulo - Brazil
[2] Univ Sao Paulo, Sch Pharmaceut Sci, Clin Chem & Toxicol Dept, Skin Biol Lab, BR-05508000 Sao Paulo - Brazil
Número total de Afiliações: 2
|
| Tipo de documento: | Artigo Científico |
| Fonte: | NANOMATERIALS; v. 10, n. 12 DEC 2020. |
| Citações Web of Science: | 0 |
| Resumo | |
The development of products for topical applications requires analyses of their skin effects before they are destined for the market. At present, the ban on animal use in several tests makes the search for in vitro models (such as artificial skin) necessary to characterize the risks involved. In this work, tissue engineering concepts were used to manufacture collagen-free three-dimensional scaffolds for cell growth and proliferation. Two different human skin models-reconstructed human epidermis and full-thickness skin-were developed from electrospun scaffolds using synthetic polymers such as polyethylene terephthalate, polybutylene terephthalate, and nylon 6/6. After the construction of these models, their histology was analyzed by H\&E staining and immunohistochemistry. The results revealed a reconstructed epidermal tissue, duly stratified, obtained from the nylon scaffold. In this model, the presence of proteins involved in the epidermis stratification process (cytokeratin 14, cytokeratin 10, involucrin, and loricrin) was confirmed by immunohistochemistry and Western blot analysis. The nylon reconstructed human epidermis model's applicability was evaluated as a platform to perform irritation and corrosion tests. Our results demonstrated that this model is a promising platform to assess the potential of dermal irritation/corrosion of chemical products. (AU) | |
| Processo FAPESP: | 11/21442-6 - Polímeros sintéticos e naturais aplicados à engenharia de tecido |
| Beneficiário: | Luiz Henrique Catalani |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 18/13492-2 - Arcabouços sintéticos e naturais aplicados à medicina regenerativa |
| Beneficiário: | Luiz Henrique Catalani |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 17/04926-6 - Melanoma e quimiorresistência: modelos in vitro e in silico para explorar alvos terapêuticos |
| Beneficiário: | Silvya Stuchi Maria-Engler |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 19/14527-7 - Bioimpressão de pele como plataforma para testes alternativos de segurança e eficácia |
| Beneficiário: | Silvya Stuchi Maria-Engler |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |