Biochemical and histopathological responses in peripubertal male rats exposed to agrochemicals isolated or in combination: A multivariate data analysis study

Autor(es)

Garcia Aranha, Maria Luiza ^[1] ; Garcia, Mariana Simoes ^[1] ; de Carvalho Cavalcante, Deborah Navit ^[1] ; Gomes Silva, Ana Priscila ^[1] ; Fontes, Mayana Karoline ^[2] ; Gusso-Choueri, Paloma Kachel ^[2] ; Choueri, Rodrigo Brasil ^[3] ; Perobelli, Juliana Elaine ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo UNIFESP, Dept Ciencias Mar, Expt Toxicol Lab, Santos, SP - Brazil

^[2] Univ Estadual Paulista UNESP, Nucleo Estudos Poluicao & Ecotoxicol Aquat, Campus Litoral Paulista, Sao Vicente, SP - Brazil

^[3] Univ Fed Sao Paulo UNIFESP, Dept Ciencias Mar, Santos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Toxicology; v. 447, JAN 15 2021.
• Citações Web of Science: 0

Resumo

The widespread use of agrochemicals results in the exposure of the general human population, including children, to several of these chemicals simultaneously. In the present preclinical study, it was investigated the hepatic damages caused by exposure to acephate, carbendazim and mancozeb when administered alone or in different combinations (binary and ternary). Juvenile male Wistar rats were exposed to agrochemicals from postnatal day 53, by gavage. The doses of agrochemicals applied here were determined from previous studies whose results showed no signs of systemic toxicity. All exposures provoked a significant increase in DNA damage (except for acephate alone) and activation of the xenobiotic biotransformation system (except for the ternary mixture). Interestingly, the ternary mixture did not exhibit an exacerbation in adverse effects caused by agrochemicals isolated or in binary combination, even though they are sharing genotoxicity damage induction as a common toxicity pathway. Conversely, some effects observed for isolated or binary combinations of agrochemicals were not observed for ternary combination, suggesting a chemical interaction that could imply antagonism character. Using a multivariate data analysis approach, exposure to isolated agrochemicals were related to a group of adverse effects characterized by hepatic lesion and the attempt of the tissue to mobilize defense cells and increase mitotic rates to minimize damages. Binary mixtures also share similarities in relation to the effects they exhibited, mainly a moderate to high increase in the GST activity and in histopathological alterations suggesting that binary combinations trigger an increased response of the mechanism of xenobiotics biotransformation. Together, obtained results bring important insights regarding adverse effects and possible interaction of the three agrochemicals whose residues are commonly detected in agro-food products. (AU)

• Processo FAPESP: 16/24033-3 - Estudo ecotoxicológico e avaliação do risco ambiental da cocaína em ecossistemas marinhos
• Beneficiário: Mayana Karoline Fontes
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 17/23063-9 - Efeitos reprodutivos, endócrinos, citotóxicos e genotóxicos dos agroquímicos acefato, carbendazim e mancozebe isolados ou em mistura: estudo de toxicidade juvenil em ratos
• Beneficiário: Juliana Elaine Perobelli
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 19/04588-9 - Efeitos reprodutivos, endócrinos, citotóxicos e genotóxicos dos agroquímicos acefato, carbendazim e mancozebe isolados ou em mistura: estudo de toxicidade juvenil em ratos
• Beneficiário: Maria Luiza Garcia Aranha
• Modalidade de apoio: Bolsas no Brasil - Programa Capacitação - Treinamento Técnico
• Processo FAPESP: 17/04970-5 - Relações entre concentrações de metais no sedimento, bioacumulação e respostas tóxicas em p. vivipara: subsídios para avaliação de risco ambiental
• Beneficiário: Paloma Kachel Gusso Choueri
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado