Biocompatible silver nanoparticles incorporated in acrylic resin for dental application inhibit Candida albicans biofilm

Autor(es)

Takamiya, Aline Satie ^[1] ; Monteiro, Douglas Roberto ^[2] ; Gorup, Luiz Fernando ^[3] ; Silva, Ebele Adaobi ^[4] ; de Camargo, Emerson Rodriques ^[3] ; Gomes-Filho, Joao Eduardo ^[5] ; Penha de Oliveira, Sandra Helena ^[6] ; Barbosa, Debora Barros ^[7]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista UNESP, Dept Diag & Surg, Aracatuba Dent Sch, Aracatuba, SP - Brazil

^[2] Univ Estadual Paulista UNESP, Aracatuba Dent Sch, Dept Pediat Dent & Publ Hlth Dent, Aracatuba, SP - Brazil

^[3] Fed Univ Sao Carlos UFSCar, LIEC Dept Chem, Sao Carlos, SP - Brazil

^[4] Sao Paulo State Univ, Ribeirao Preto Dent Sch, Dept Dent Mat & Prosthodont, Ribeirao Preto, SP - Brazil

^[5] Univ Estadual Paulista UNESP, Aracatuba Dent Sch, Dept Endodont, Aracatuba, SP - Brazil

^[6] Univ Estadual Paulista UNESP, Aracatuba Dent Sch, Dept Basic Sci, Aracatuba, SP - Brazil

^[7] Univ Estadual Paulista UNESP, Aracatuba Dent Sch, Dept Dent Mat & Prosthodont, Aracatuba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Materials Science & Engineering C-Materials for Biological Applications; v. 118, JAN 2021.
• Citações Web of Science: 1

Resumo

Background: Although silver nanoparticles (SNP) have proven antimicrobial activity against different types of microorganisms, the effect of SNP incorporation into acrylic resin to control Candida albicans biofilm formation aiming at the prevention of Candida-associated denture stomatitis has not yet been fully elucidated. Objectives: This study aimed to evaluate the antimicrobial effect of an acrylic resin containing SNP on C. albicans biofilm growth, the flexural strength of this material and tissue reaction in the subcutaneous connective tissue of rats to SNP. Method: SNP were synthesized through silver nitrate reduction by sodium citrate. Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) were used to verify the size and colloidal stability. SNP were added to acrylic resin monomer (Lucitone 550) at 0.05, 0.5 and 5 vol%. The antimicrobial effect against C. albicans (ATCC 10231) was investigated by the enumeration of colony-forming units (CFUs) and SEM. The threepoint bending test was performed to analyze the flexural strength. Tissue reaction was evaluated after 7 and 60 days of implantation in the connective tissue of Wistar rats. Results: Spherical particles of 5 and 10 nm were obtained. SNP at 0.05 and 0.5% incorporated into acrylic resin was effective in reducing C. albicans biofilm growth (p < .001). SEM revealed that the material was able to disrupt C. albicans biofilm formation and did not reduce the flexural strength compared to control (p > .05). The inflammatory response observed 60 days after implantation SNP in the subcutaneous tissue was similar to control. Conclusion: It was concluded that SNP addition at 0.05 and 0.5% into acrylic resin exhibited antimicrobial effects against C. albicans biofilm, did not interfere in the flexural strength and may be considered biocompatible. (AU)

• Processo FAPESP: 10/05788-7 - Avaliação da resposta tecidual e da citotoxicidade de soluções coloidais de nanopartículas de prata
• Beneficiário: Aline Satie Takamiya
• Modalidade de apoio: Bolsas no Brasil - Doutorado