| Texto completo | |
| Autor(es): |
de Araujo, Marcelo Valdemir
[1]
;
Dos Santos Junior, Samuel Rodrigues
[1]
;
Nosanchuk, Joshua D.
[2, 3, 4, 5]
;
Taborda, Carlos Pelleschi
[1, 6]
Número total de Autores: 4
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, BR-05508000 Sao Paulo - Brazil
[2] Albert Einstein Coll Med, Dept Med, Div Infect Dis, Bronx, NY 10461 - USA
[3] Albert Einstein Coll Med, Dept Microbiol, Div Infect Dis, Bronx, NY 10461 - USA
[4] Albert Einstein Coll Med, Dept Immunol, Div Infect Dis, Bronx, NY 10461 - USA
[5] Montefiore Med Ctr, Bronx, NY 10461 - USA
[6] Univ Sao Paulo, Fac Med, Inst Med Trop Sao Paulo LIM53, Dept Dermatol, BR-4023062 Sao Paulo - Brazil
Número total de Afiliações: 6
|
| Tipo de documento: | Artigo Científico |
| Fonte: | JOURNAL OF FUNGI; v. 6, n. 4 DEC 2020. |
| Citações Web of Science: | 0 |
| Resumo | |
The peptide P10 is a vaccine candidate for Paracoccidioidomycosis, a systemic mycosis caused by fungal species of the genus Paracoccidioides spp. We have previously shown that peptide P10 vaccination, in the presence of several different adjuvants, induced a protective cellular immune response mediated by CD4(+) Th-1 lymphocytes that was associated with the increased production of IFN-gamma in mice challenged with a virulent isolate of Paracoccidoides brasiliensis. Cationic liposomes formulated with dioctadecyldimethylammonium and trehalose dibehenate (DDA/TDB, termed also CAF01-cationic adjuvant formulation) have been developed for safe administration in humans and CAF01 liposomes are utilized as an adjuvant for modulating a robust Th-1/Th-17 cellular response. We evaluated the efficacy of the adsorption of peptide P10 to CAF01 cationic liposomes and used the generated liposomes to vaccinate C57Bl/6 mice infected with P. brasiliensis. Our results showed that P10 was efficiently adsorbed onto CAF01 liposomes. The vaccination of infected mice with cationic liposomes formulated with DDA/TDB 250/50 mu g/mL and 20 mu g of P10 induced an effective cellular immune response with increased levels of Th-17 cytokines, which correlated with significant decreases in the fungal burdens in lungs and protective granulomatous tissue responses. Hence, cationic liposomes of DDA/TDB 250/50 mu g/mL with 20 mu g of P10 are a promising therapeutic for safely and effectively improving the treatment of paracoccidioidomycosis. (AU) | |
| Processo FAPESP: | 16/08730-6 - Patogenicidade fúngica: efeito do tabagismo, resposta imune e a modulação vacinal na paracoccidioidomicose e na histoplasmose |
| Beneficiário: | Carlos Pelleschi Taborda |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |