Synthesis of multifunctional chlorhexidine-doped thin films for titanium-based implant materials

Autor(es):Mostrar menos -

Matos, Adaias Oliveira ^{[1, 2]} ; de Almeida, Amanda Bandeira ^[1] ; Beline, Thamara ^[1] ; Tonon, Caroline C. ^[3] ; Viana Casarin, Renato Correa ^[1] ; Windsor, Lester Jack ^[2] ; Duarte, Simone ^[3] ; Nociti Jr, Francisco Humberto ; Rangel, Elidiane Cipriano ^[4] ; Gregory, Richard L. ^[2] ; Ricardo Barao, Valentim Adelino ^[5]

Afiliação do(s) autor(es)

^[1] Univ Campinas UNICAMP, Piracicaba Dent Sch, Dept Prosthodont & Periodontol, Piracicaba, SP - Brazil

^[2] Indiana Univ, Sch Dent, Dept Biomed Sci & Comprehens Care, Indianapolis, IN - USA

^[3] Indiana Univ, Sch Dent, Purdue Univ Indianapolis, Dept Cariol Operat Dent & Dent Publ Hlth, Indianapolis, IN - USA

^[4] Sao Paulo State Univ UNESP, Sci & Technol Inst Sorocaba ICTS, Lab Technol Plasmas LaPTec, Sorocaba, SP - Brazil

^[5] Nociti Jr, Jr., Francisco Humberto, Univ Campinas UNICAMP, Piracicaba Dent Sch, Dept Prosthodont & Periodontol, Piracicaba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Materials Science & Engineering C-Materials for Biological Applications; v. 117, DEC 2020.
• Citações Web of Science: 0

Resumo

Our goal was to create bio-functional chlorhexidine (CHX)-doped thin films on commercially pure titanium (cpTi) discs using the glow discharge plasma approach. Different plasma deposition times (50, 35 and 20 min) were used to create bio-functional surfaces based on silicon films with CHX that were compared to the control groups {[}no CHX and bulk cpTi surface (machined)]. Physico-chemical and biological characterizations included: 1. Morphology, roughness, elemental chemical composition, film thickness, contact angle and surface free energy; 2. CHX-release rate; 3. Antibacterial effect on Streptococcus sanguinis biofilms at 24, 48 and 72 h; 4. Cytotoxicity and metabolic activity using fibroblasts cell culture (NIH-F3T3 cells) at 1, 2, 3 and 4 days; 5. Protein expression by NIH-F3T3 cells at 1, 2, 3 and 4 days; and 6. Co-culture assay of fibroblasts cells and S. sanguinis to assess live and dead cells on the confocal laser scanning microscopy, mitochondrial activity (XTT), membrane leakage (LDH release), and metabolic activity (WST-1 assay) at 1, 2 and 3 days of co-incubation. Data analysis showed that silicon films, with or without CHX coated cpTi discs, increased surface wettability and free energy (p < 0.05) without affecting surface roughness. CHX release was maintained over a 22-day period and resulted in a significant inhibition of biofilm growth (p 0.05) at 48 and 72 h of biofilm formation for 50 min and 20 min of plasma deposition time groups, respectively. In general, CHX treatment did not significantly affect NIH-F3T3 cell viability (p 0.05). Co-culture demonstrated that CHX-doped film did not affect the metabolic activity, cytotoxicity and viability of fibroblasts cells (p 0.05). Altogether, the findings of the current study support the conclusion that silicon films added with CHX can be successfully created on titanium discs and have the potential to affect bacterial growth and inflammatory markers without affecting cell viability/proliferation rates. (AU)

• Processo FAPESP: 16/06117-5 - Desenvolvimento de filmes de hexametildisiloxano incorporados com clorexidina na superfície de titânio: potencial antimicrobiano e citotoxicidade
• Beneficiário: Adaias Oliveira Matos
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 17/21894-0 - Potencial antimicrobiano e de biocompatibilidade de filmes de hexametildisiloxano incorporados a clorhexidina em superfície de titânio usando um modelo de epitélio oral gengival co-infectado reconstituído in vitro
• Beneficiário: Adaias Oliveira Matos
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado