Integrated use of biomarkers to evaluate the reproductive physiology of Astyanax fasciatus and Hoplias malabaricus males (Teleostei: Characiformes) in polluted reservoirs

Autor(es)

Escalante-Rojas, Maria C. ^[1] ; Tolussi, Carlos E. ^[2] ; Gomes, Aline D. ^[1] ; Munoz-Penuela, Marcela ^[1] ; Brambila-Souza, Gabriela ^[3] ; Branco, Giovana S. ^[3] ; Moreira, Renata G. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biociencias, Dept Fisiol, Lab Metab & Reprod Organismas Aquat, Rua Matao, Trav 14, 321, BR-05508090 Sao Paulo, SP - Brazil

^[2] Univ Anhembi Morumbi, Escola Ciencias Saude, R Dr Almeida Lima, 1134, Parque Mooca, BR-03164000 Sao Paulo, SP - Brazil

^[3] Univ Estadual Paulista, Ctr Aquicultura CAUNESP, V Acesso Prof Paulo Donato Castelane S-N, BR-14884900 Jaboticabal, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY; v. 208, JAN 15 2021.
• Citações Web of Science: 3

Resumo

The reproductive physiology of fish can be changed by the presence of pollutants in the water, which act as endocrine disrupting compounds (EDC). We evaluated the impacts of water contaminants in polluted reservoirs acting as possible EDC on the reproductive physiology of Astyanax fasciatus and Hoplias malabaricus males. We used biomarkers with different levels of biological organization. Hoplias malabaricus adult males were collected in the summer and winter at five different sites in the Tiete River Basin: the Ponte Nova reservoir (PN), considered a reference site due to the low anthropogenic influence; the Billings reservoir (BIL) at two different branches; and the Guarapiranga reservoir (GUA) at two different branches. Astyanax fasciatus adult males were collected at PN and BIL. BIL and GUA are subjected to great anthropogenic action. We analyzed gonadal histomorphology, testosterone (T), 11-ketotestosterone (11-KT), estradiol (E2) plasma levels, and gene expression of hepatic vitellogenin (vtgA) and pituitary follicle stimulating hormone (fsh beta). In the PN reservoir (reference), the biomarkers analyzed in both species did not differ between the periods analyzed. This is an evidence that the animals keep the same reproductive activity during both seasons. The changes in the plasma concentration of gonadal steroids in both species in polluted reservoirs suggest the presence of EDC compounds in the water and/ or adjusts of the physiological setpoint to allow the reproduction in such adverse conditions. The use of vtgA as biomarker suggests the presence of estrogenic compounds, mainly in BIL, but with a more evident response of H. malabaricus. However, even considering physiological changes, both species present testes during the maturation phase that allow the reproduction in an environment with a high degree of pollution. (AU)

• Processo FAPESP: 17/11530-1 - Ajustes fisiológicos de teleósteos tropicais em reservatórios impactados
• Beneficiário: Renata Guimarães Moreira Whitton
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/16320-7 - Impactos das mudanças climáticas e ambientais sobre a fauna: uma abordagem integrativa
• Beneficiário: Carlos Arturo Navas Iannini
• Modalidade de apoio: Auxílio à Pesquisa - Programa de Pesquisa sobre Mudanças Climáticas Globais - Temático