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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Improved in vitro and in vivo Anti-Candida albicans Activity of Cymbopogon nardus Essential Oil by Its Incorporation into a Microemulsion System

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Autor(es):
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de Toledo, Luciani Gaspar [1] ; dos Santos Ramos, Matheus Aparecido [1] ; da Silva, Patricia Bento [2] ; Rodero, Camila Fernanda [3] ; Gomes, Veridiana de Sa [1] ; da Silva, Anderson Noronha [1] ; Pavan, Fernando Rogerio [1] ; da Silva, Isabel Cristiane [1] ; Oda, Fernando Bombarda [4] ; Flumignan, Danilo Luis [5] ; dos Santos, Andre Gonzaga [4] ; Chorilli, Marius [3] ; Gottardo de Almeida, Margarete Teresa [6] ; Bauab, Tais Maria [1]
Número total de Autores: 14
Afiliação do(s) autor(es):
[1] Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, Dept Biol Sci, Campus Araraquara, BR-14800903 Sao Paulo - Brazil
[2] Univ Brasilia UnB, Inst Biol Sci, Dept Genet & Morphol, Brasilia, DF - Brazil
[3] Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, Dept Drug & Med, Sao Paulo - Brazil
[4] Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, Dept Nat Act Principles & Toxicol, Sao Paulo - Brazil
[5] Sao Paulo Fed Inst Educ Sci & Technol IFSP, Sao Paulo - Brazil
[6] Fac Med Sao Jose do Rio Preto FAMERP, Dept Infect Dis, Sao Paulo - Brazil
Número total de Afiliações: 6
Tipo de documento: Artigo Científico
Fonte: INTERNATIONAL JOURNAL OF NANOMEDICINE; v. 15, p. 10481-10497, 2020.
Citações Web of Science: 0
Resumo

Purpose: Vulvovaginal candidiasis (VVC) is an opportunistic fungal infection that adversely affects a woman's health, due to unpleasant symptoms, therapeutic challenges, and the emergence of resistant strains. The association of natural products and nanotechnology is important to improve the antifungal potential of medicinal plants. We aimed to evaluate the in vitro and in vivo anti-Candida albicans activity of unloaded (EO) and loaded (ME+EO) essential oil of Cymbopogon nardus in the microemulsion (ME). Methods: The chemical analysis of the EO was performed by gas chromatography-mass spectrometry. The ME and ME+EO were characterized by scattering, zeta potential, polarized light microscopy, rheological assays, mucoadhesiveness and transmission electronic microscopy. The in vitro antifungal activity of the EO and ME+EO were evaluated by microdilution technique. The toxicity of EO and ME+EO was analyzed on human cell line HaCat and using alternative model assay with Artemia salina. The experimental in vivo VVC was performed in female mice (C57BL/6). Results: The main compounds of the EO were found to be citronellal, geranial, geraniol, citronellol, and neral. The formulations exhibited suitable size, homogeneity, negative charge, isotropic behavior, highly organized structure, and pseudoplastic behavior, for vaginal application. TEM photomicrographs showed possible EO droplets inside the spherical structures. The EO, when loaded into the ME, exhibited an improvement in its antifungal action against C. albicans. The EO was not toxic against brine shrimp nauplii. An in vivo VVC assay showed that the use of the ME significantly improved the action of the EO, since only the ME+EO promoted the eradication of the fungal vaginal infection on the third day of treatment. Conclusion: The EO and ME+EO are promising alternatives for the control of fungal infections caused by C. albicans, once the use of nanotechnology significantly improved the antifungal action of the EO, especially in an in vivo model of VVC. (AU)

Processo FAPESP: 20/13057-4 - Atividade anti-Candida albicans in vitro e in vivo aprimorada do óleo essencial de Cymbopogon nardus pela sua incorporação em um sistema microemulsionado
Beneficiário:Taís Maria Bauab
Modalidade de apoio: Auxílio à Pesquisa - Publicações científicas - Artigo
Processo FAPESP: 16/08559-5 - Análise química e prospecção terapêutica de Syngonanthus nitens (Bong.) Ruhland e Cymbopogon nardus (L.) Rendle incorporados em sistemas nanotecnológicos aplicados ao tratamento da candidíase vulvovaginal
Beneficiário:Taís Maria Bauab
Modalidade de apoio: Auxílio à Pesquisa - Regular
Processo FAPESP: 14/50926-0 - INCT 2014: biodiversidade e produtos naturais
Beneficiário:Vanderlan da Silva Bolzani
Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático
Processo FAPESP: 15/23959-7 - Cymbopogon nardus (L.) Rendle (citronela): prospecção química-biológica do óleo essencial com destaque no estudo de biofilme e controle da candidíase vulvovaginal
Beneficiário:Luciani Gaspar de Toledo
Modalidade de apoio: Bolsas no Brasil - Doutorado