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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Diclofenac and caffeine inhibit hepatic antioxidant enzymes in the freshwater fish Astyanax altiparanae (Teleostei: Characiformes)

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Autor(es):
Munoz-Penuela, Marcela [1] ; Lo Nostro, Fabiana Laura [2, 3] ; Gomes, Aline Dal'Olio [1] ; Tolussi, Carlos Eduardo [4] ; Branco, Giovana Souza [1] ; Silva Pinheiro, Joao Paulo [1] ; Andrade de Godoi, Filipe Guilherme [1] ; Moreira, Renata Guimaraes [1]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Lab Metab & Reprod Organisms Aquat, Inst Biociencias, Dept Fisiol, Sao Paulo - Brazil
[2] Univ Buenos Aires, Fac Ciencias Exactas & Nat, Dept Biodiversidad & Biol Expt, Lab Ecotoxicol Acuat, Ciudad Univ, Buenos Aires, DF - Argentina
[3] CONICET UBA, IBBEA, Ciudad Univ, Buenos Aires, DF - Argentina
[4] Univ Anhembi Morumbi, Campus Mooca, Sao Paulo - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY; v. 240, FEB 2021.
Citações Web of Science: 2
Resumo

Although concentrations of pharmaceutical compounds in aquatic ecosystems are low, they can cause toxic effects on organisms. The aim of this study was to evaluate the effects of diclofenac (DCF), a non-steroidal anti-inflammatory drug, and caffeine (CAF), a central nervous system stimulant, both alone or combined, in Astyanax altiparanae males under acute exposure (96 h), measuring neurotoxicity biomarkers, antioxidant response and damage at biochemical and cellular levels. DCF concentration in water, separated and combined, was 3.08 mg L-1 and that of CAF was 9.59 mg L-1. To assess neurotoxicity, brain and muscle acetylcholinesterase (AChE) activities were measured. To evaluate oxidative stress, the enzymatic activities of superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) and glutathione S-transferase (GST), as well as lipoperoxidation (LPO), were analyzed in liver and gills. Activity of hepatic cyclooxygenase (COX) was also evaluated. Genotoxicity was assessed in blood using comet assay and micronucleus test, as well as nuclear abnormalities. DCF and CAF, alone or combined, had neither effect on AChE activity, nor in the activity of SOD, CAT, GPx and GST in gills. In liver, DCF inhibited SOD and GPx activity, CAF inhibited CAT activity, the mixture inhibited SOD and GST activity; although only fish exposed to CAF showed increased hepatic LPO. Under these experimental conditions, no effect on COX activity was observed, nor cytotoxic and genotoxic damage. The most pronounced effects were caused by the drugs separately, since both compounds altered the enzymes, but only CAF triggered LPO, showing more harmful effects. (AU)

Processo FAPESP: 17/11530-1 - Ajustes fisiológicos de teleósteos tropicais em reservatórios impactados
Beneficiário:Renata Guimarães Moreira Whitton
Modalidade de apoio: Auxílio à Pesquisa - Regular
Processo FAPESP: 14/16320-7 - Impactos das mudanças climáticas e ambientais sobre a fauna: uma abordagem integrativa
Beneficiário:Carlos Arturo Navas Iannini
Modalidade de apoio: Auxílio à Pesquisa - Programa de Pesquisa sobre Mudanças Climáticas Globais - Temático