Three-stage repeated-batch immobilized cell fermentation to produce butanol from non-detoxified sugarcane bagasse hemicellulose hydrolysates

Autor(es)

Chacon, Suranny Jimenez ^[1] ; Matias, Gabriela ^[1] ; Ezeji, Thaddeus Chukwuemeka ^[2] ; Maciel Filho, Rubens ^[1] ; Mariano, Adriano Pinto ^[1]

Afiliação do(s) autor(es)

^[1] Univ Campinas UNICAMP, Sch Chem Engn, Lab Optimizat Design & Adv Control, Fermentat Div LOPCA Ferm, Campinas, SP - Brazil

^[2] Ohio State Univ, Ohio State Agr Res & Dev Ctr, Dept Anim Sci, Wooster, OH - USA

• Tipo de documento: Artigo Científico
• Fonte: Bioresource Technology; v. 321, FEB 2021.
• Citações Web of Science: 0

Resumo

To enable the production of butanol with undiluted, non-detoxified sugarcane bagasse hemicellulose hydrolysates, this study developed a three-staged repeated-batch immobilized cell fermentation in which the efficiency of a 3D-printed nylon carrier to passively immobilize Clostridium saccharoperbutylacetonicum DSM 14923 was compared with sugarcane bagasse. The first stage consisted of sugarcane molasses fermentation, and in the second stage, non-detoxified sugarcane bagasse hemicellulose hydrolysates (SBHH) was pulse-fed to sugarcane molasses fermentation. In the next four batches, immobilized cells were fed with undiluted SBHH supplemented with molasses, and SBHH-derived xylose accounted for approximately 50% of the sugars. Bagasse was a superior carrier, and the average xylose utilization (33%) was significantly higher than the treatment with the 3D-printed carrier (16%). Notably, bagasse allowed for 43% of the butanol to be SBHH-derived. Overall, cell immobilization on lignocellulosic materials can be an efficient strategy to produce butanol from repeated-batch fermentation of non-detoxified hemicellulose hydrolysates. (AU)

• Processo FAPESP: 15/20630-4 - Desenvolvimento integrado de biorrefinaria e planta de bioetanol de cana-de-açúcar com emissão zero de CO2: rotas para converter recursos renováveis em bioprodutos e bioeletricidade
• Beneficiário: Rubens Maciel Filho
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 18/25925-0 - Produção de n-butanol a partir da fermentação de hidrolisado hemicelulósico do bagaço da cana-de-açúcar com imobilização celular
• Beneficiário: Gabriela Matias
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 18/23983-3 - Rotas para produção de pneus a partir da cana-de-açúcar: conversão eficiente de açúcares em precursores de 1,3-butadieno e avaliação técnico-econômica ambiental
• Beneficiário: Adriano Pinto Mariano
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Regular